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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Human severe sepsis cytokine mixture increases β2-integrin-dependent polymorphonuclear leukocyte adhesion to cerebral microvascular endothelial cells in vitro
|
|---|---|
| Published in |
Critical Care, December 2015
|
| DOI | 10.1186/s13054-015-0883-z |
| Pubmed ID | |
| Authors |
Chris Blom, Brittany L Deller, Douglas D Fraser, Eric K Patterson, Claudio M Martin, Bryan Young, Patricia C Liaw, Payam Yazdan-Ashoori, Angelica Ortiz, Brian Webb, Greg Kilmer, David E Carter, Gediminas Cepinskas |
| Abstract |
Sepsis-associated encephalopathy (SAE) is a state of acute brain dysfunction in response to a systemic infection. We propose that systemic inflammation during sepsis causes increased adhesion of leukocytes to the brain microvasculature, resulting in blood brain barrier dysfunction. Thus, our objectives were (1) to measure inflammatory analytes in plasma of severe sepsis patients to create an experimental cytokine mixture (CM), and (2) to use this CM to investigate the activation and interactions of polymorphonuclear leukocytes (PMN) and human cerebrovascular endothelial cells (hCMEC/D3) in vitro. The concentrations of 41 inflammatory analytes were quantified in plasma obtained from 20 severe sepsis patients and 20 age-/sex-matched healthy controls employing an antibody microarray. Two CMs were prepared to mimic severe sepsis (SSCM) and control (CCM), and these CMs were then used for PMN and hCMEC/D3 stimulation in vitro. PMN adhesion to hCMEC/D3 was assessed under conditions of flow (shear stress 0.7 dyn/cm(2)). Eight inflammatory analytes elevated in plasma obtained from severe sepsis patients were used to prepare SSCM and CCM. Stimulation of PMN with SSCM led to a marked increase in PMN adhesion to hCMEC/D3, as compared to CCM. PMN adhesion was abolished with neutralizing antibodies to either β2 (CD18), αL/β2 (CD11α/CD18; LFA-1) or αM/β2 (CD11β/CD18; Mac-1) integrins. In addition, immune-neutralization of the endothelial (hCMEC/D3) cell adhesion molecule, ICAM-1 (CD54) also suppressed PMN adhesion. Human SSCM up-regulates PMN pro-adhesive phenotype and promotes PMN adhesion to cerebrovascular endothelial cells through a β2-integrin-ICAM-1-dependent mechanism. PMN adhesion to the brain microvasculature may contribute to SAE. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Puerto Rico | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Unknown | 51 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 21% |
| Student > Ph. D. Student | 8 | 15% |
| Student > Master | 8 | 15% |
| Professor | 6 | 12% |
| Student > Doctoral Student | 4 | 8% |
| Other | 10 | 19% |
| Unknown | 5 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 24 | 46% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Agricultural and Biological Sciences | 4 | 8% |
| Immunology and Microbiology | 2 | 4% |
| Business, Management and Accounting | 2 | 4% |
| Other | 7 | 13% |
| Unknown | 7 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 April 2015.
All research outputs
#15,755,393
of 25,394,764 outputs
Outputs from Critical Care
#5,132
of 6,558 outputs
Outputs of similar age
#209,838
of 395,593 outputs
Outputs of similar age from Critical Care
#428
of 466 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,558 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 20.8. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 395,593 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 466 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.