Title |
Molecular approaches to determine the multiplicity of Plasmodium infections
|
---|---|
Published in |
Malaria Journal, April 2018
|
DOI | 10.1186/s12936-018-2322-5 |
Pubmed ID | |
Authors |
Daibin Zhong, Cristian Koepfli, Liwang Cui, Guiyun Yan |
Abstract |
Multiplicity of infection (MOI), also termed complexity of infection (COI), is defined as the number of genetically distinct parasite strains co-infecting a single host, which is an important indicator of malaria epidemiology. PCR-based genotyping often underestimates MOI. Next generation sequencing technologies provide much more accurate and genome-wide characterization of polyclonal infections. However, complete haplotype characterization of multiclonal infections remains a challenge due to PCR artifacts and sequencing errors, and requires efficient computational tools. In this review, the advantages and limitations of current molecular approaches to determine multiplicity of malaria parasite infection are discussed. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Belgium | 1 | 17% |
United States | 1 | 17% |
Unknown | 4 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 2 | 33% |
Science communicators (journalists, bloggers, editors) | 2 | 33% |
Scientists | 1 | 17% |
Members of the public | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 133 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 25 | 19% |
Researcher | 24 | 18% |
Student > Master | 19 | 14% |
Student > Bachelor | 12 | 9% |
Student > Doctoral Student | 8 | 6% |
Other | 22 | 17% |
Unknown | 23 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 40 | 30% |
Agricultural and Biological Sciences | 21 | 16% |
Immunology and Microbiology | 13 | 10% |
Medicine and Dentistry | 11 | 8% |
Nursing and Health Professions | 3 | 2% |
Other | 17 | 13% |
Unknown | 28 | 21% |