Title |
Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
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Published in |
Parasites & Vectors, April 2018
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DOI | 10.1186/s13071-018-2853-1 |
Pubmed ID | |
Authors |
Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Hassan Hakimi, Tatsunori Masatani, Patrick Vudriko, Seung-Hun Lee, Shin-ichiro Kawazu, Junya Yamagishi, Xuenan Xuan |
Abstract |
Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 16% |
Student > Ph. D. Student | 5 | 16% |
Student > Doctoral Student | 3 | 9% |
Student > Master | 3 | 9% |
Student > Bachelor | 2 | 6% |
Other | 6 | 19% |
Unknown | 8 | 25% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 9 | 28% |
Biochemistry, Genetics and Molecular Biology | 6 | 19% |
Medicine and Dentistry | 2 | 6% |
Agricultural and Biological Sciences | 2 | 6% |
Immunology and Microbiology | 2 | 6% |
Other | 2 | 6% |
Unknown | 9 | 28% |