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Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni

Overview of attention for article published in Parasites & Vectors, April 2018
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Title
Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni
Published in
Parasites & Vectors, April 2018
DOI 10.1186/s13071-018-2853-1
Pubmed ID
Authors

Mingming Liu, Paul Franck Adjou Moumouni, Masahito Asada, Hassan Hakimi, Tatsunori Masatani, Patrick Vudriko, Seung-Hun Lee, Shin-ichiro Kawazu, Junya Yamagishi, Xuenan Xuan

Abstract

Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 16%
Student > Ph. D. Student 5 16%
Student > Doctoral Student 3 9%
Student > Master 3 9%
Student > Bachelor 2 6%
Other 6 19%
Unknown 8 25%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 9 28%
Biochemistry, Genetics and Molecular Biology 6 19%
Medicine and Dentistry 2 6%
Agricultural and Biological Sciences 2 6%
Immunology and Microbiology 2 6%
Other 2 6%
Unknown 9 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2018.
All research outputs
#20,483,282
of 23,045,021 outputs
Outputs from Parasites & Vectors
#4,889
of 5,511 outputs
Outputs of similar age
#287,605
of 326,557 outputs
Outputs of similar age from Parasites & Vectors
#150
of 173 outputs
Altmetric has tracked 23,045,021 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,511 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 173 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.