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AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms

Overview of attention for article published in Biotechnology for Biofuels, April 2018
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Title
AC-202, a highly effective fluorophore for the visualization of lipid droplets in green algae and diatoms
Published in
Biotechnology for Biofuels, April 2018
DOI 10.1186/s13068-018-1117-9
Pubmed ID
Authors

Seddik Harchouni, Ben Field, Benoît Menand

Abstract

Lipid-specific live cell dyes are an important tool for the study of algal lipid metabolism, the monitoring of lipid production, and the identification of algal strains with high lipid yields. Nile Red and BODIPY have emerged as the principal dyes for these purposes. However, they suffer from a number of shortcomings including for specificity, penetration, interference from chlorophyll autofluorescence, and incompatibility with widely used genetically encoded reporters in the green and blue regions of the spectrum such as the green fluorescent protein and the red fluorescent protein. We tested a new blue fluorescent dye, AC-202, in both the green algae Chlamydomonas reinhardtii and the pennate diatom Phaeodactylum tricornutum. We show that AC-202 is effective in both algae and that after minimal sample preparation, it can label lipid droplets induced by nitrogen starvation or by inhibitors of the TOR (target of rapamycin) kinase. We found that AC-202 benefits from a low background signal and is therefore more sensitive than BODIPY for semiquantitative in vivo fluorescence measurements. Finally, a co-staining experiment indicated that AC-202 can be used for multicolor imaging in combination with both red and green fluorophores. AC-202 is an alternative and highly effective fluorophore for algal research that resolves drawbacks encountered with other neutral lipid dyes. AC-202 can be used to rapidly and sensitively visualize lipid droplets, and will contribute to the identification of metabolic and signaling pathways involved in lipid droplet formation, monitoring lipid production, and in the development of screens for algal strains suitable for biofuel production.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 14%
Other 4 14%
Student > Master 4 14%
Researcher 4 14%
Professor 2 7%
Other 5 18%
Unknown 5 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 25%
Biochemistry, Genetics and Molecular Biology 7 25%
Linguistics 1 4%
Environmental Science 1 4%
Chemical Engineering 1 4%
Other 3 11%
Unknown 8 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2018.
All research outputs
#9,324,653
of 15,220,640 outputs
Outputs from Biotechnology for Biofuels
#636
of 1,149 outputs
Outputs of similar age
#155,913
of 277,498 outputs
Outputs of similar age from Biotechnology for Biofuels
#1
of 1 outputs
Altmetric has tracked 15,220,640 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,149 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,498 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them