Outdoor biting by Anopheles mosquitoes on Bioko Island does not currently impact on malaria control

John Bradley, Jo Lines, Godwin Fuseini, Christopher Schwabe, Feliciano Monti, Michel Slotman, Daniel Vargas, Guillermo Garcia, Dianna Hergott, Immo Kleinschmidt

There have been many recent reports that the rate of outdoor biting by malaria vectors has increased. This study examined the impact this might have on malaria transmission by assessing the association between exposure to outdoor bites and malaria infection on Bioko Island, Equatorial Guinea. Responses to questions about time spent outside the previous night from a malaria indicator were combined with human landing catch measurements of hourly rates of outdoor and indoor biting for the whole island to estimate the number of outdoor and indoor bites received by each survey respondent and related to their RDT measured malaria infection status. The average number of bites received per person per night was estimated as 3.51 in total, of which 0.690 (19.7%) would occur outdoors. Malaria infection was not significantly higher in individuals who reported spending time outside between 7 pm and 6 am the previous night compared to those not spending time outside in both adults (18.9% vs 17.4%, p = 0.20) and children (29.2% vs 27.1%, p = 0.20). Malaria infection in neither adults (p = 0.56) nor in children (p = 0.12) was associated with exposure to outdoor bites, even after adjusting for confounders. Malaria vector mosquitoes in Bioko do bite humans outdoors, and this has the potential to reduce the effectiveness of vector control. However, outdoor biting is currently not a major factor influencing the malaria burden, mainly because more than 95% of the population are indoors during the middle of the night, which is the peak biting period for malaria vector mosquitoes. The majority of resources should remain with control measures that target indoor biting and resting such as LLINs and IRS.