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Adipose cells promote resistance of breast cancer cells to trastuzumab-mediated antibody-dependent cellular cytotoxicity

Overview of attention for article published in Breast Cancer Research, April 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (60th percentile)

Mentioned by

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3 tweeters
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2 Facebook pages

Citations

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76 Dimensions

Readers on

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93 Mendeley
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Title
Adipose cells promote resistance of breast cancer cells to trastuzumab-mediated antibody-dependent cellular cytotoxicity
Published in
Breast Cancer Research, April 2015
DOI 10.1186/s13058-015-0569-0
Pubmed ID
Authors

Minh Ngoc Duong, Aurore Cleret, Eva-Laure Matera, Kamel Chettab, Doriane Mathé, Sandrine Valsesia-Wittmann, Béatrice Clémenceau, Charles Dumontet

Abstract

Trastuzumab has been used in the treatment of human epidermal growth factor receptor 2 (HER2)-expressing breast cancer but its efficacy is limited due to de novo or acquired resistance. Although many mechanisms have been proposed to explain the resistance to trastuzumab, little is known concerning the role of the tumor microenvironment. Given the importance of antibody-dependent cell-mediated cytotoxicity (ADCC) in the antitumor effect of trastuzumab and the abundance of adipose tissue in breast, we investigated the impact of adipocytes on ADCC. We set up a co-culture system to study the effect of adipocytes on ADCC in vitro. The results were validated in vivo in xenograft mice. We found that adipocytes, as well as preadipocytes, inhibited trastuzumab-mediated ADCC in HER2-expressing breast cancer cells via the secretion of soluble factors. The inhibition of ADCC was not due to a titration or a degradation of the antibody. We found that adipose cells decreased the secretion of interferon-gamma by natural killer cells, but did not alter their cytotoxicity. Pre-incubation of breast cancer cells with the conditioned medium derived from adipocytes reduced the sensitivity of cancer cells to ADCC. Using a transcriptomic approach, we found that cancer cells undergo major modifications when exposed to adipocyte-conditioned medium. Importantly, breast tumor grafted next to lipoma displayed resistance to trastuzumab in xenograft mouse models. Collectively, our findings underline the importance of adipose tissue in the resistance to trastuzumab, and suggest that approaches targeting the adipocyte-cancer cell crosstalk may help sensitize cancer cells to trastuzumab-based therapy.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Austria 1 1%
Unknown 91 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 31%
Researcher 21 23%
Student > Master 12 13%
Student > Bachelor 6 6%
Student > Doctoral Student 4 4%
Other 10 11%
Unknown 11 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 24%
Biochemistry, Genetics and Molecular Biology 21 23%
Medicine and Dentistry 20 22%
Immunology and Microbiology 2 2%
Engineering 2 2%
Other 7 8%
Unknown 19 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 May 2015.
All research outputs
#7,985,353
of 15,135,311 outputs
Outputs from Breast Cancer Research
#928
of 1,614 outputs
Outputs of similar age
#89,574
of 230,630 outputs
Outputs of similar age from Breast Cancer Research
#1
of 1 outputs
Altmetric has tracked 15,135,311 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,614 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.1. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 230,630 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them