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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes
|
|---|---|
| Published in |
Genome Medicine, April 2018
|
| DOI | 10.1186/s13073-018-0542-5 |
| Pubmed ID | |
| Authors |
Praneeth Reddy Devulapally, Jörg Bürger, Thorsten Mielke, Zoltán Konthur, Hans Lehrach, Marie-Laure Yaspo, Jörn Glökler, Hans-Jörg Warnatz |
| Abstract |
Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies. |
X Demographics
The data shown below were collected from the profiles of 15 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 7% |
| Germany | 1 | 7% |
| Mexico | 1 | 7% |
| Denmark | 1 | 7% |
| Austria | 1 | 7% |
| Spain | 1 | 7% |
| United Kingdom | 1 | 7% |
| Unknown | 8 | 53% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 60% |
| Scientists | 6 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 76 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 29% |
| Student > Master | 7 | 9% |
| Student > Ph. D. Student | 6 | 8% |
| Professor > Associate Professor | 5 | 7% |
| Professor | 4 | 5% |
| Other | 10 | 13% |
| Unknown | 22 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 21% |
| Agricultural and Biological Sciences | 12 | 16% |
| Immunology and Microbiology | 11 | 14% |
| Medicine and Dentistry | 5 | 7% |
| Chemistry | 3 | 4% |
| Other | 4 | 5% |
| Unknown | 25 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2018.
All research outputs
#3,823,151
of 23,884,161 outputs
Outputs from Genome Medicine
#779
of 1,481 outputs
Outputs of similar age
#72,372
of 329,747 outputs
Outputs of similar age from Genome Medicine
#19
of 26 outputs
Altmetric has tracked 23,884,161 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,481 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.5. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 329,747 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.