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Determining the role of IL-4 induced neuroinflammation in microglial activity and amyloid-β using BV2 microglial cells and APP/PS1 transgenic mice

Overview of attention for article published in Journal of Neuroinflammation, March 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Mentioned by

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1 news outlet
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2 X users
patent
1 patent

Citations

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83 Dimensions

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127 Mendeley
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Title
Determining the role of IL-4 induced neuroinflammation in microglial activity and amyloid-β using BV2 microglial cells and APP/PS1 transgenic mice
Published in
Journal of Neuroinflammation, March 2015
DOI 10.1186/s12974-015-0243-6
Pubmed ID
Authors

Clare H Latta, Tiffany L Sudduth, Erica M Weekman, Holly M Brothers, Erin L Abner, Gabriel J Popa, Michael D Mendenhall, Floracita Gonzalez-Oregon, Kaitlyn Braun, Donna M Wilcock

Abstract

Microglia are considered the resident immune cells of the central nervous system (CNS). In response to harmful stimuli, an inflammatory reaction ensues in which microglia are activated in a sequenced spectrum of pro- and antiinflammatory phenotypes that are akin to the well-characterized polarization states of peripheral macrophages. A "classically" activated M1 phenotype is known to eradicate toxicity. The transition to an "alternatively" activated M2 phenotype encompasses neuroprotection and repair. In recent years, inflammation has been considered an accompanying pathology in response to the accumulation of extracellular amyloid-β (Aβ) in Alzheimer's disease (AD). This study aimed to drive an M2a-biased immune phenotype with IL-4 in vitro and in vivo and to determine the subsequent effects on microglial activation and Aβ pathology. In vitro, exogenous IL-4 was applied to BV2 microglial cell cultures to evaluate the temporal progression of microglial responses. In vivo, intracranial injections of an adeno-associate-virus (AAV) viral vector were performed to assess long-term expression of IL-4 in the frontal cortex and hippocampus of Aβ-depositing, APP/PS1 transgenic mice. Quantitative real-time PCR was used to assess the fold change in expression of biomarkers representing each of the microglial phenotypes in both the animal tissue and the BV2 cells. ELISAs quantified IL-4 expression and Aβ levels. Histological staining permitted quantification of microglial and astrocytic activity. Both in vitro and in vivo models showed an enhanced M2a phenotype, and the in vivo model revealed a trend toward a decreased trend in Aβ deposition. In summary, this study offers insight into the therapeutic potential of microglial immune response in AD.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 127 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Unknown 126 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 27 21%
Student > Bachelor 22 17%
Student > Master 20 16%
Researcher 14 11%
Professor > Associate Professor 6 5%
Other 13 10%
Unknown 25 20%
Readers by discipline Count As %
Neuroscience 31 24%
Agricultural and Biological Sciences 20 16%
Biochemistry, Genetics and Molecular Biology 19 15%
Pharmacology, Toxicology and Pharmaceutical Science 8 6%
Medicine and Dentistry 8 6%
Other 13 10%
Unknown 28 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2017.
All research outputs
#2,337,217
of 22,800,560 outputs
Outputs from Journal of Neuroinflammation
#329
of 2,629 outputs
Outputs of similar age
#31,291
of 257,838 outputs
Outputs of similar age from Journal of Neuroinflammation
#1
of 44 outputs
Altmetric has tracked 22,800,560 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,629 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 257,838 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.