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Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches

Overview of attention for article published in BMC Plant Biology, April 2015
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  • Above-average Attention Score compared to outputs of the same age (57th percentile)

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3 tweeters

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Title
Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches
Published in
BMC Plant Biology, April 2015
DOI 10.1186/s12870-015-0485-6
Pubmed ID
Authors

Rhonda C Foley, Jose C Jimenez-Lopez, Lars G Kamphuis, James K Hane, Su Melser, Karam B Singh

Abstract

The major proteins in lupin seeds are conglutins that have primary roles in supplying carbon, sulphur and nitrogen and energy for the germinating seedling. They fall into four families; α, β, γ and δ. Interest in these conglutins is growing as family members have been shown to have beneficial nutritional and pharmaceutical properties. An in-depth transcriptome and draft genome from the narrow-leafed lupin (NLL; Lupinus angustifolius) variety, Tanjil, were examined and 16 conglutin genes were identified. Using RNAseq data sets, the structure and expression of these 16 conglutin genes were analysed across eight lupin varieties from five lupin species. Phylogenic analysis suggest that the α and γ conglutins diverged prior to lupin speciation while β and δ members diverged both prior and after speciation. A comparison of the expression of the 16 conglutin genes was performed, and in general the conglutin genes showed similar levels of RNA expression among varieties within species, but quite distinct expression patterns between lupin species. Antibodies were generated against the specific conglutin families and immunoblot analyses were used to compare the levels of conglutin proteins in various tissues and during different stages of seed development in NLL, Tanjil, confirming the expression in the seed. This analysis showed that the conglutins were expressed highly at the mature seed stage, in all lupin species, and a range of polypeptide sizes were observed for each conglutin family. This study has provided substantial information on the complexity of the four conglutin families in a range of lupin species in terms of their gene structure, phylogenetic relationships as well as their relative RNA and protein abundance during seed development. The results demonstrate that the majority of the heterogeneity of conglutin polypeptides is likely to arise from post-translational modification from a limited number of precursor polypeptides rather than a large number of different genes. Overall, the results demonstrate a high degree of plasticity for conglutin expression during seed development in different lupin species.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Switzerland 1 2%
Egypt 1 2%
Unknown 60 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 17%
Researcher 10 16%
Student > Master 8 13%
Student > Bachelor 7 11%
Professor 4 6%
Other 6 10%
Unknown 17 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 35%
Biochemistry, Genetics and Molecular Biology 15 24%
Chemistry 2 3%
Environmental Science 1 2%
Immunology and Microbiology 1 2%
Other 3 5%
Unknown 19 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2015.
All research outputs
#9,146,208
of 16,638,522 outputs
Outputs from BMC Plant Biology
#733
of 2,429 outputs
Outputs of similar age
#98,340
of 232,564 outputs
Outputs of similar age from BMC Plant Biology
#1
of 1 outputs
Altmetric has tracked 16,638,522 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,429 research outputs from this source. They receive a mean Attention Score of 3.1. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 232,564 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them