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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Proteomic analysis of porcine mesenchymal stem cells derived from bone marrow and umbilical cord: implication of the proteins involved in the higher migration capability of bone marrow mesenchymal stem cells
|
|---|---|
| Published in |
Stem Cell Research & Therapy, April 2015
|
| DOI | 10.1186/s13287-015-0061-x |
| Pubmed ID | |
| Authors |
Lei Huang, Chenguang Niu, Belinda Willard, Weimin Zhao, Lan Liu, Wei He, Tianwen Wu, Shulin Yang, Shutang Feng, Yulian Mu, Lemin Zheng, Kui Li |
| Abstract |
Mesenchymal stem cells (MSCs) have the ability to proliferate in vivo with a large variety of differentiation potentials, and therefore, they are widely used as an ideal material for cell therapy. MSCs derived from pig and human sources are similar in many aspects, such as cell immunophenotype and functional characteristics. However, differences in proteomics and the molecular mechanisms of cell functions between porcine bone marrow MSCs (BM-MSCs) and umbilical cord MSCs (UC-MSCs) are largely unknown. To our knowledge, MSCs collected from different tissue have specific phenotype and differentiation ability in response to microenvironment, known as a niche. Porcine BM-MSCs and UC-MSCs were evaluated with flow cytometric and adipogenic and osteogenic differentiation analyses. We utilized isobaric tagging, for relative and absolute quantitation (iTRAQ), combined with LC-MS/MS, to identify differentially expressed proteins (DEPs) between these two types of MSCs. KEGG pathway and phenotype analyses were used to understand the links between cell migration ability and DEPs. Two separate iTRAQ experiments were conducted, identifying 95 DEPs (95% confidence interval). Five of these proteins were verified by western blotting. These 95 DEPs were classified in terms of biological regulation, metabolic process, developmental process, immune system process, reproduction, death, growth, signaling, localization, response to stimulus, biological adhesion and cellular component organization. Our study is the first to show results indicating that porcine BM-MSCs have a higher migration capability than UC-MSCs. Finally, one of the DEPs, Vimentin, was verified to have a positive role in MSC migration. These results represent the first attempt to use proteomics specifically targeted to porcine MSCs of different tissues. The identified components should help reveal a variety of tissue-specific functions in tissue-derived MSCs populations and could serve as important tools for the regeneration of particular tissues in future stem cell-based tissue engineering studies using animal models. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 20% |
| Researcher | 8 | 15% |
| Student > Master | 7 | 13% |
| Student > Bachelor | 4 | 7% |
| Professor | 3 | 6% |
| Other | 8 | 15% |
| Unknown | 13 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Agricultural and Biological Sciences | 7 | 13% |
| Medicine and Dentistry | 7 | 13% |
| Neuroscience | 3 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 8 | 15% |
| Unknown | 19 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2020.
All research outputs
#6,952,799
of 22,800,560 outputs
Outputs from Stem Cell Research & Therapy
#679
of 2,418 outputs
Outputs of similar age
#82,312
of 264,074 outputs
Outputs of similar age from Stem Cell Research & Therapy
#27
of 73 outputs
Altmetric has tracked 22,800,560 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 2,418 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,074 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.