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Profiling of differentially expressed genes in sheep T lymphocytes response to an artificial primary Haemonchus contortus infection

Overview of attention for article published in Parasites & Vectors, April 2015
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Title
Profiling of differentially expressed genes in sheep T lymphocytes response to an artificial primary Haemonchus contortus infection
Published in
Parasites & Vectors, April 2015
DOI 10.1186/s13071-015-0844-z
Pubmed ID
Authors

Yi Yang, Qian-Jin Zhou, Xue-Qiu Chen, Bao-Long Yan, Xiao-Lu Guo, Hong-Li Zhang, Ai-Fang Du

Abstract

Haemonchus contortus is a common bloodsucking nematode causing widespread economic loss in agriculture. Upon H. contortus infection, a series of host responses is elicited, especially those related to T lymphocyte immunity. Existing studies mainly focus on the general immune responses of sheep T lymphocyte to H. contortus, lacking investigations at the molecular level. The objective of this study was to obtain a systematic transcriptional profiling of the T lymphocytes in H. contortus primary-infected sheep. Nematode-free sheep were orally infected once with H. contortus L3s. T lymphocyte samples were collected from the peripheral blood of 0, 3, 30 and 60 days post infection (dpi) infected sheep. Microarrays were used to compare gene transcription levels between samples. Quantitative RT-PCR was employed to validate the microarray data. Gene Ontology and KEGG pathway analysis were utilized for the annotation of differentially expressed genes. Our microarray data was consistent with qPCR results. From microarrays, 853, 242 and 42 differentially expressed genes were obtained in the 3d vs. 0d, 30d vs. 0d and 60d vs. 0d comparison groups, respectively. Gene Ontology and KEGG pathway analysis indicated that these genes were involved in metabolism, signaling, cell growth and immune system processes. Functional analysis of significant differentially expressed genes, such as SLC9A3R2, ABCB9, COMMD4, SUGT1, FCER1G, GSK3A, PAK4 and FCER2, revealed a crucial association with cellular homeostasis maintenance and immune response. Our data suggested that maintaining both effective immunological response and natural cellular activity are important for T lymphocytes in fighting against H. contortus infection. Our results provide a substantial list of candidate genes in sheep T lymphocytes response to H. contortus infection, and contribute novel insights into a general immune response upon infection.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 43%
Student > Master 4 14%
Researcher 3 11%
Student > Doctoral Student 2 7%
Professor 1 4%
Other 3 11%
Unknown 3 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 39%
Biochemistry, Genetics and Molecular Biology 5 18%
Veterinary Science and Veterinary Medicine 3 11%
Medicine and Dentistry 1 4%
Chemistry 1 4%
Other 0 0%
Unknown 7 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2015.
All research outputs
#14,812,533
of 19,211,930 outputs
Outputs from Parasites & Vectors
#3,309
of 4,901 outputs
Outputs of similar age
#158,563
of 239,883 outputs
Outputs of similar age from Parasites & Vectors
#1
of 1 outputs
Altmetric has tracked 19,211,930 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,901 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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