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The lycopene β-cyclase plays a significant role in provitamin A biosynthesis in wheat endosperm

Overview of attention for article published in BMC Plant Biology, May 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (67th percentile)
  • Good Attention Score compared to outputs of the same age and source (79th percentile)

Mentioned by

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2 X users
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1 Wikipedia page

Citations

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26 Dimensions

Readers on

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49 Mendeley
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1 CiteULike
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Title
The lycopene β-cyclase plays a significant role in provitamin A biosynthesis in wheat endosperm
Published in
BMC Plant Biology, May 2015
DOI 10.1186/s12870-015-0514-5
Pubmed ID
Authors

Jian Zeng, Cheng Wang, Xi Chen, Mingli Zang, Cuihong Yuan, Xiatian Wang, Qiong Wang, Miao Li, Xiaoyan Li, Ling Chen, Kexiu Li, Junli Chang, Yuesheng Wang, Guangxiao Yang, Guangyuan He

Abstract

Lycopene β-cyclase (LCYB) is a key enzyme catalyzing the biosynthesis of β-carotene, the main source of provitamin A. However, there is no documented research about this key cyclase gene's function and relationship with β-carotene content in wheat. Therefore, the objectives of this study were to clone TaLCYB and characterize its function and relationship with β-carotene biosynthesis in wheat grains. We also aimed to obtain more information about the endogenous carotenoid biosynthetic pathway and thus provide experimental support for carotenoid metabolic engineering in wheat. In the present study, a lycopene β-cyclase gene, designated TaLCYB, was cloned from the hexaploid wheat cultivar Chinese Spring. The cyclization activity of the encoded protein was demonstrated by heterologous complementation analysis. The TaLCYB gene was expressed differentially in different tissues of wheat. Although TaLCYB had a higher expression level in the later stages of grain development, the β-carotene content still showed a decreasing tendency. The expression of TaLCYB in leaves was dramatically induced by strong light and the β-carotene content variation corresponded with changes of TaLCYB expression. A post-transcriptional gene silencing strategy was used to down-regulate the expression of TaLCYB in transgenic wheat, resulting in a decrease in the content of β-carotene and lutein, accompanied by the accumulation of lycopene to partly compensate for the total carotenoid content. In addition, changes in TaLCYB expression also affected the expression of several endogenous carotenogenic genes to varying degrees. Our results suggest that TaLCYB is a genuine lycopene cyclase gene and plays a crucial role in β-carotene biosynthesis in wheat. Our attempt to silence it not only contributes to elucidating the mechanism of carotenoid accumulation in wheat but may also help in breeding wheat varieties with high provitamin A content through RNA interference (RNAi) to block specific carotenogenic genes in the wheat endosperm.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Russia 1 2%
Unknown 48 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 18%
Student > Master 9 18%
Student > Ph. D. Student 6 12%
Student > Bachelor 4 8%
Professor > Associate Professor 4 8%
Other 7 14%
Unknown 10 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 45%
Biochemistry, Genetics and Molecular Biology 10 20%
Chemical Engineering 1 2%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Psychology 1 2%
Other 2 4%
Unknown 12 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2016.
All research outputs
#6,954,391
of 22,805,349 outputs
Outputs from BMC Plant Biology
#552
of 3,244 outputs
Outputs of similar age
#82,598
of 264,549 outputs
Outputs of similar age from BMC Plant Biology
#10
of 54 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 3,244 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,549 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.