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Granulosa cell-derived induced pluripotent stem cells exhibit pro-trophoblastic differentiation potential

Overview of attention for article published in Stem Cell Research & Therapy, February 2015
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Title
Granulosa cell-derived induced pluripotent stem cells exhibit pro-trophoblastic differentiation potential
Published in
Stem Cell Research & Therapy, February 2015
DOI 10.1186/s13287-015-0005-5
Pubmed ID
Authors

Ching-Yu Chuang, Mei-Chi Huang, Hsin-Fu Chen, Li-Hui Tseng, Chun-Ying Yu, Lee Stone, Hsiang-Po Huang, Hong-Nerng Ho, Hung-Chih Kuo

Abstract

Human induced pluripotent stem cells (hiPSCs) have been derived from various somatic cell types. Granulosa cells, a group of cells which surround oocytes and are obtained from the (normally discarded) retrieved egg follicles of women undergoing infertility treatment, are a possible cell source for induced pluripotent stem cell (iPSC) generation. Here, we explored the possibility of using human granulosa cells as a donor cell type for iPSC reprogramming, and compared granulosa cell-derived iPSCs (iGRAs) with those derived from other cell sources, to determine the potential ability of iGRA differentiation. Granulosa cells were collected from egg follicles retrieved from women undergoing infertility treatment. After short-term culture, the granulosa cells derived from different patients were mixed in culture, and infected with retroviruses encoding reprogramming factors. The resulting iPSC clones were selected and subjected to microsatellite DNA analysis to determine their parental origin. IGRAs were subjected to RT-PCR, immunofluorescence staining, and in vitro and in vivo differentiation assays to further establish their pluripotent characteristics. Microsatellite DNA analysis was used to demonstrate that hiPSCs with different parental origins can be simultaneously reprogrammed by retroviral transfection of a mixed human granulosa cell population obtained from multiple individuals. The iGRAs resemble human embryonic stem cells (hESCs) in many respects, including morphological traits, growth requirements, gene and marker expression profiles, and in vitro and in vivo developmental propensities. We also demonstrate that the iGRAs express low levels of NLRP2, and differentiating iGRAs possess a biased differentiation potential toward the trophoblastic lineage. Although NLRP2 knockdown in hESCs promotes trophoblastic differentiation of differentiating hESCs, it does not result in exit from pluripotency. These results imply that NLRP2 may play a role in regulating the trophoblastic differentiation of human pluripotent stem cells. These findings provide a means of generating iPSCs from multiple granulosa cell populations with different parental origins. The ability to generate iPSCs from granulosa cells not only enables modeling of infertility-associated disease, but also provides a means of identifying potential clinical interventions through iPSC-based drug screening.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Bulgaria 1 3%
South Africa 1 3%
Unknown 31 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 21%
Student > Master 5 15%
Student > Doctoral Student 4 12%
Student > Postgraduate 4 12%
Student > Bachelor 3 9%
Other 8 24%
Unknown 3 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 29%
Agricultural and Biological Sciences 9 26%
Medicine and Dentistry 4 12%
Chemical Engineering 1 3%
Veterinary Science and Veterinary Medicine 1 3%
Other 3 9%
Unknown 6 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2016.
All research outputs
#17,758,492
of 22,805,349 outputs
Outputs from Stem Cell Research & Therapy
#1,583
of 2,418 outputs
Outputs of similar age
#173,724
of 255,566 outputs
Outputs of similar age from Stem Cell Research & Therapy
#34
of 49 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,418 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,566 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.