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Horizontal transfer of exosomal microRNAs transduce apoptotic signals between pancreatic beta-cells

Overview of attention for article published in Cell Communication and Signaling, March 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

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5 X users
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2 patents

Citations

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124 Dimensions

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134 Mendeley
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1 CiteULike
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Title
Horizontal transfer of exosomal microRNAs transduce apoptotic signals between pancreatic beta-cells
Published in
Cell Communication and Signaling, March 2015
DOI 10.1186/s12964-015-0097-7
Pubmed ID
Authors

Claudiane Guay, Véronique Menoud, Sophie Rome, Romano Regazzi

Abstract

Diabetes mellitus is a common metabolic disorder characterized by dysfunction of insulin-secreting pancreatic beta-cells. MicroRNAs are important regulators of beta-cell activities. These non-coding RNAs have recently been discovered to exert their effects not only inside the cell producing them but, upon exosome-mediated transfer, also in other recipient cells. This novel communication mode remains unexplored in pancreatic beta-cells. In the present study, the microRNA content of exosomes released by beta-cells in physiological and physiopathological conditions was analyzed and the biological impact of their transfer to recipient cells investigated. Exosomes were isolated from the culture media of MIN6B1 and INS-1 derived 832/13 beta-cell lines and from mice, rat or human islets. Global profiling revealed that the microRNAs released in MIN6B1 exosomes do not simply reflect the content of the cells of origin. Indeed, while a subset of microRNAs was preferentially released in exosomes others were selectively retained in the cells. Moreover, exposure of MIN6B1 cells to inflammatory cytokines changed the release of several microRNAs. The dynamics of microRNA secretion and their potential transfer to recipient cells were next investigated. As a proof-of-concept, we demonstrate that if cel-miR-238, a C. Elegans microRNA not present in mammalian cells, is expressed in MIN6B1 cells a fraction of it is released in exosomes and is transferred to recipient beta-cells. Furthermore, incubation of untreated MIN6B1 or mice islet cells in the presence of microRNA-containing exosomes isolated from the culture media of cytokine-treated MIN6B1 cells triggers apoptosis of recipient cells. In contrast, exosomes originating from cells not exposed to cytokines have no impact on cell survival. Apoptosis induced by exosomes produced by cytokine-treated cells was prevented by down-regulation of the microRNA-mediating silencing protein Ago2 in recipient cells, suggesting that the effect is mediated by the non-coding RNAs. Taken together, our results suggest that beta-cells secrete microRNAs that can be transferred to neighboring beta-cells. Exposure of donor cells to pathophysiological conditions commonly associated with diabetes modifies the release of microRNAs and affects survival of recipient beta-cells. Our results support the concept that exosomal microRNAs transfer constitutes a novel cell-to-cell communication mechanism regulating the activity of pancreatic beta-cells.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 134 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
Luxembourg 1 <1%
Canada 1 <1%
Unknown 131 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 27 20%
Student > Ph. D. Student 23 17%
Student > Bachelor 17 13%
Researcher 14 10%
Student > Doctoral Student 7 5%
Other 24 18%
Unknown 22 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 34 25%
Agricultural and Biological Sciences 30 22%
Medicine and Dentistry 28 21%
Immunology and Microbiology 5 4%
Nursing and Health Professions 2 1%
Other 6 4%
Unknown 29 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2023.
All research outputs
#3,724,729
of 25,059,640 outputs
Outputs from Cell Communication and Signaling
#102
of 1,364 outputs
Outputs of similar age
#46,329
of 269,493 outputs
Outputs of similar age from Cell Communication and Signaling
#1
of 12 outputs
Altmetric has tracked 25,059,640 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,364 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,493 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.