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Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans

Overview of attention for article published in BMC Microbiology, March 2015
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Title
Kinin release from human kininogen by 10 aspartic proteases produced by pathogenic yeast Candida albicans
Published in
BMC Microbiology, March 2015
DOI 10.1186/s12866-015-0394-8
Pubmed ID
Authors

Andrzej Kozik, Mariusz Gogol, Oliwia Bochenska, Justyna Karkowska-Kuleta, Natalia Wolak, Wojciech Kamysz, Wataru Aoki, Mitsuyoshi Ueda, Alexander Faussner, Maria Rapala-Kozik

Abstract

Candida albicans yeast produces 10 distinct secreted aspartic proteases (Saps), which are some of the most important virulence factors of this pathogenic fungus. One of the suggested roles of Saps is their deregulating effect on various proteolytic cascades that constitute the major homeostatic systems in human hosts, including blood coagulation, fibrinolysis, and kallikrein-kinin systems. This study compared the characteristics of the action of all 10 Saps on human kininogens, which results in generating proinflammatory bradykinin-related peptides (kinins). Recombinant forms of Saps, heterologously overexpressed in Pichia pastoris were applied. Except for Sap7 and Sap10, all Saps effectively cleaved the kininogens, with the highest hydrolytic activity toward the low-molecular-mass form (LK). Sap1-6 and 8 produced a biologically active kinin-Met-Lys-bradykinin-and Sap3 was exceptional in terms of the kinin-releasing yield (>60% LK at pH 5.0 after 24 hours). Des-Arg(1)-bradykinin was released from LK by Sap9 at a comparably high yield, but this peptide was assumed to be biologically inactive because it was unable to interact with cellular B2-type kinin receptors. However, the collaborative actions of Sap9 and Sap1, -2, -4-6, and -8 on LK rerouted kininogen cleavage toward the high-yield release of the biologically active Met-Lys-bradykinin. Our present results, together with the available data on the expression of individual SAP genes in candidal infection models, suggest a biological potential of Saps to produce kinins at the infection foci. The kinin release during candidiasis can involve predominant and complementary contributions of two different Sap3- and Sap9-dependent mechanisms.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 3%
Unknown 28 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 21%
Student > Master 5 17%
Student > Ph. D. Student 5 17%
Student > Postgraduate 3 10%
Student > Bachelor 1 3%
Other 3 10%
Unknown 6 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 41%
Biochemistry, Genetics and Molecular Biology 8 28%
Veterinary Science and Veterinary Medicine 1 3%
Immunology and Microbiology 1 3%
Social Sciences 1 3%
Other 0 0%
Unknown 6 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2015.
All research outputs
#4,207,382
of 5,058,381 outputs
Outputs from BMC Microbiology
#818
of 1,013 outputs
Outputs of similar age
#139,379
of 169,055 outputs
Outputs of similar age from BMC Microbiology
#29
of 32 outputs
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