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Crystal structure of the fibre head domain of bovine adenovirus 4, a ruminant atadenovirus

Overview of attention for article published in Virology Journal, May 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)

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11 tweeters
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1 Facebook page
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1 Wikipedia page

Citations

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6 Dimensions

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21 Mendeley
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Title
Crystal structure of the fibre head domain of bovine adenovirus 4, a ruminant atadenovirus
Published in
Virology Journal, May 2015
DOI 10.1186/s12985-015-0309-1
Pubmed ID
Authors

Thanh H. Nguyen, Márton Z. Vidovszky, Mónika Z. Ballmann, Marta Sanz-Gaitero, Abhimanyu K. Singh, Balázs Harrach, Mária Benkő, Mark J. van Raaij

Abstract

In adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other adenovirus capsid proteins and leads to a productive infection. Although many fibre head structures are known for human mastadenoviruses, not many animal adenovirus fibre head structures have been determined, especially not from those belonging to adenovirus genera other than Mastadenovirus. We constructed an expression vector for the fibre head domain from a ruminant atadenovirus, bovine adenovirus 4 (BAdV-4), consisting of amino acids 414-535, expressed the protein in Escherichia coli, purified it by metal affinity and cation exchange chromatography and crystallized it. The structure was solved using single isomorphous replacement plus anomalous dispersion of a mercury derivative and refined against native data that extended to 1.2 Å resolution. Like in other adenoviruses, the BAdV-4 fibre head monomer contains a beta-sandwich consisting of ABCJ and GHID sheets. The topology is identical to the fibre head of the other studied atadenovirus, snake adenovirus 1 (SnAdV-1), including the alpha-helix in the DG-loop, despite of them having a sequence identity of only 15 %. There are also differences which may have implications for ligand binding. Beta-strands G and H are longer and differences in several surface-loops and surface charge are observed. Chimeric adenovirus fibres have been used to retarget adenovirus-based anti-cancer and gene therapy vectors. Ovine adenovirus 7 (OAdV-7), another ruminant atadenovirus, is intensively tested as a basis for such a vector. Here, we present the high-resolution atomic structure of the BAdV-4 fibre head domain, the second atadenovirus fibre head structure known and the first of an atadenovirus that infects a mammalian host. Future research should focus on the receptor-binding properties of these fibre head domains.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 19%
Researcher 4 19%
Student > Bachelor 3 14%
Professor > Associate Professor 2 10%
Student > Master 1 5%
Other 3 14%
Unknown 4 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 29%
Veterinary Science and Veterinary Medicine 2 10%
Chemistry 2 10%
Agricultural and Biological Sciences 2 10%
Unspecified 1 5%
Other 2 10%
Unknown 6 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2019.
All research outputs
#2,256,185
of 17,165,110 outputs
Outputs from Virology Journal
#196
of 2,542 outputs
Outputs of similar age
#37,359
of 240,118 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
Altmetric has tracked 17,165,110 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,542 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 20.6. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,118 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them