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Mendeley readers
Attention Score in Context
| Title |
A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
|
|---|---|
| Published in |
Virology Journal, May 2018
|
| DOI | 10.1186/s12985-018-0983-x |
| Pubmed ID | |
| Authors |
Fang-zhou Qiu, Xin-xin Shen, Meng-chuan Zhao, Li Zhao, Su-xia Duan, Chen Chen, Ju-Ju Qi, Gui-xia Li, Le Wang, Zhi-shan Feng, Xue-jun Ma |
| Abstract |
Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children. Molecular typing of HAdV is not routinely performed in clinical diagnostic laboratories as it is time-consuming and labor-intensive. In the present study, we developed a triplex quantitative real-time PCR assay (tq-PCR) in a single closed tube for differential detection and quantitative analysis of HAdV serotypes 2, 3 and 7. The sensitivity, specificity, reproducibility and clinical performance of tq-PCR were evaluated. The analytical sensitivity of the tq-PCR was 100 copies/reaction for each of HAdV serotypes 2, 3 and 7, and no cross-reaction with other common respiratory viruses or HAdV serotypes 1,4,5,6,31,55 and 57 was observed. The coefficients of variation (CV) of intra-assay and inter-assay were between 0.6% to 3.6%. Of 138 previously-defined HAdV-positive nasopharyngeal aspirates samples tested, the detection agreement between tq-PCR and nested PCR was 96.38% (133/138). The proposed tq-PCR assay is a sensitive, specific and reproducible method and has the potential for clinical use in the rapid and differential detection and quantitation of HAdV serotypes 2, 3 and 7. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 18% |
| Student > Bachelor | 4 | 14% |
| Researcher | 3 | 11% |
| Student > Postgraduate | 2 | 7% |
| Professor | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 9 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 18% |
| Biochemistry, Genetics and Molecular Biology | 4 | 14% |
| Immunology and Microbiology | 2 | 7% |
| Chemistry | 2 | 7% |
| Agricultural and Biological Sciences | 1 | 4% |
| Other | 4 | 14% |
| Unknown | 10 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 May 2018.
All research outputs
#18,606,163
of 23,047,237 outputs
Outputs from Virology Journal
#2,456
of 3,063 outputs
Outputs of similar age
#253,054
of 326,328 outputs
Outputs of similar age from Virology Journal
#43
of 52 outputs
Altmetric has tracked 23,047,237 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,063 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.7. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 326,328 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.