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Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures

Overview of attention for article published in BMC Musculoskeletal Disorders, May 2015
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Title
Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures
Published in
BMC Musculoskeletal Disorders, May 2015
DOI 10.1186/s12891-015-0588-0
Pubmed ID
Authors

Kayleigh Wong, Fangui Sun, Guy Trudel, Paola Sebastiani, Odette Laneuville

Abstract

Contractures of the knee joint cause disability and handicap. Recovering range of motion is recognized by arthritic patients as their preference for improved health outcome secondary only to pain management. Clinical and experimental studies provide evidence that the posterior knee capsule prevents the knee from achieving full extension. This study was undertaken to investigate the dynamic changes of the joint capsule transcriptome during the progression of knee joint contractures induced by immobilization. We performed a microarray analysis of genes expressed in the posterior knee joint capsule following induction of a flexion contracture by rigidly immobilizing the rat knee joint over a time-course of 16 weeks. Fold changes of expression values were measured and co-expressed genes were identified by clustering based on time-series analysis. Genes associated with immobilization were further analyzed to reveal pathways and biological significance and validated by immunohistochemistry on sagittal sections of knee joints. Changes in expression with a minimum of 1.5 fold changes were dominated by a decrease in expression for 7732 probe sets occurring at week 8 while the expression of 2251 probe sets increased. Clusters of genes with similar profiles of expression included a total of 162 genes displaying at least a 2 fold change compared to week 1. Functional analysis revealed ontology categories corresponding to triglyceride metabolism, extracellular matrix and muscle contraction. The altered expression of selected genes involved in the triglyceride biosynthesis pathway; AGPAT-9, and of the genes P4HB and HSP47, both involved in collagen synthesis, was confirmed by immunohistochemistry. Gene expression in the knee joint capsule was sensitive to joint immobility and provided insights into molecular mechanisms relevant to the pathophysiology of knee flexion contractures. Capsule responses to immobilization was dynamic and characterized by modulation of at least three reaction pathways; down regulation of triglyceride biosynthesis, alteration of extracellular matrix degradation and muscle contraction gene expression. The posterior knee capsule may deploy tissue-specific patterns of mRNA regulatory responses to immobilization. The identification of altered expression of genes and biochemical pathways in the joint capsule provides potential targets for the therapy of knee flexion contractures.

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The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 22%
Other 6 16%
Student > Doctoral Student 4 11%
Student > Ph. D. Student 3 8%
Researcher 2 5%
Other 4 11%
Unknown 10 27%
Readers by discipline Count As %
Medicine and Dentistry 11 30%
Agricultural and Biological Sciences 4 11%
Nursing and Health Professions 3 8%
Biochemistry, Genetics and Molecular Biology 2 5%
Veterinary Science and Veterinary Medicine 1 3%
Other 4 11%
Unknown 12 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 May 2015.
All research outputs
#14,226,014
of 22,807,037 outputs
Outputs from BMC Musculoskeletal Disorders
#2,125
of 4,042 outputs
Outputs of similar age
#138,352
of 266,750 outputs
Outputs of similar age from BMC Musculoskeletal Disorders
#31
of 54 outputs
Altmetric has tracked 22,807,037 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,042 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.1. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,750 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.