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Rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of M2 protein linked to flagellin in plants using viral vectors

Overview of attention for article published in BMC Biotechnology, May 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

news
1 news outlet
twitter
1 tweeter

Citations

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43 Dimensions

Readers on

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59 Mendeley
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Title
Rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of M2 protein linked to flagellin in plants using viral vectors
Published in
BMC Biotechnology, May 2015
DOI 10.1186/s12896-015-0164-6
Pubmed ID
Authors

Eugenia S. Mardanova, Roman Y. Kotlyarov, Victor V. Kuprianov, Liudmila A. Stepanova, Liudmila M. Tsybalova, George P. Lomonosoff, Nikolai V. Ravin

Abstract

The extracellular domain of matrix protein 2 (M2e) of influenza A virus is a promising target for the development of a universal vaccine against influenza because M2e sequences are highly conserved among human influenza A strains. However, native M2e is poorly immunogenic, but its immunogenicity can be increased by delivery in combination with adjuvants or carrier particles. It was previously shown that fusion of M2e to bacterial flagellin, the ligand for Toll-like receptor (TLR) 5 and powerful mucosal adjuvant, significantly increases the immunogenicity and protective capacity of M2e. In this study, we report for the first time the transient expression in plants of a recombinant protein Flg-4M comprising flagellin of Salmonella typhimurium fused to four tandem copies of the M2e peptide. The chimeric construct was expressed in Nicotiana benthamiana plants using either the self-replicating potato virus X (PVX) based vector, pA7248AMV-GFP, or the cowpea mosaic virus (CPMV)-derived expression vector, pEAQ-HT. The highest expression level up to 30 % of total soluble protein (about 1 mg/g of fresh leaf tissue) was achieved with the PVX-based expression system. Intranasal immunization of mice with purified Flg-4M protein induced high levels of M2e-specific serum antibodies and provided protection against lethal challenge with influenza virus. This study confirms the usefulness of flagellin as a carrier of M2e and its relevance for the production of M2e-based candidate influenza vaccines in plants.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 2 3%
Spain 1 2%
Canada 1 2%
Unknown 55 93%

Demographic breakdown

Readers by professional status Count As %
Student > Master 15 25%
Student > Ph. D. Student 13 22%
Researcher 7 12%
Student > Postgraduate 4 7%
Student > Bachelor 4 7%
Other 11 19%
Unknown 5 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 36%
Biochemistry, Genetics and Molecular Biology 8 14%
Immunology and Microbiology 7 12%
Medicine and Dentistry 6 10%
Pharmacology, Toxicology and Pharmaceutical Science 4 7%
Other 7 12%
Unknown 6 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2015.
All research outputs
#1,275,458
of 11,801,205 outputs
Outputs from BMC Biotechnology
#84
of 668 outputs
Outputs of similar age
#34,902
of 228,658 outputs
Outputs of similar age from BMC Biotechnology
#21
of 39 outputs
Altmetric has tracked 11,801,205 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 668 research outputs from this source. They receive a mean Attention Score of 4.6. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,658 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.