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Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

Overview of attention for article published in BMC Pregnancy and Childbirth, May 2018
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Title
Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides
Published in
BMC Pregnancy and Childbirth, May 2018
DOI 10.1186/s12884-018-1774-5
Pubmed ID
Authors

Eliane Saori Otaguiri, Ana Elisa Belotto Morguette, Alexandre Tadachi Morey, Eliandro Reis Tavares, Gilselena Kerbauy, Rosângela S. L. de Almeida Torres, Mauricio Chaves Júnior, Maria Cristina Bronharo Tognim, Viviane Monteiro Góes, Marco Aurélio Krieger, Marcia Regina Eches Perugini, Lucy Megumi Yamauchi, Sueli Fumie Yamada-Ogatta

Abstract

Streptococcus agalactiae or Group B Streptococcus (GBS) remains the leading cause of infections in newborns worldwilde. Prenatal GBS screening of pregnant women for vaginal-rectal colonization is recommended in many countries to manage appropriate intrapartum antimicrobial prophylaxis for those identified as carriers. In this study, a novel melting-curve based multiplex real-time PCR assay for the simultaneous detection of GBS and macrolide and lincosamide resistance markers was developed. The usefulness of the assay was evaluated for rapid and accurate prenatal GBS screening. One hundred two pregnant women who were at 35-37 weeks of gestation were enrolled in this study. The analytical performance of the multiplex real-time PCR was first tested using a panel of reference and clinical bacterial and fungal strains. To test the clinical performance, vaginal-rectal swabs were obtained from pregnant women who were seen at the teaching hospital for regular prenatal care. The results of real-time were compared with those obtained from microbiological analyses. The real-time PCR assay showed 100% specificity and a limit of detection of 104 colony forming units equivalent per reaction. The prevalence of GBS colonization among the population studied was 15.7% (16/102) based on a positive culture and the real-time PCR results. Agreement between the two assays was found for 11 (68.75%) GBS colonized women. Using the culture-based results as a reference, the multiplex real-time PCR had a sensitivity of 91.7% (11/12, CI 59.7-99.6%), a specificity of 95.5% (86/90, CI 89.8-98.7%), a positive predictive value of 73.3% (11/15, CI 44.8-91.1%) and a negative predictive value of 98.9% (86/87, CI 92.9-99.9%). The multiplex real-time PCR is a rapid, affordable and sensitive assay for direct detection of GBS in vaginal-rectal swabs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 58 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 10%
Student > Ph. D. Student 5 9%
Student > Doctoral Student 5 9%
Student > Bachelor 5 9%
Student > Master 4 7%
Other 11 19%
Unknown 22 38%
Readers by discipline Count As %
Medicine and Dentistry 13 22%
Agricultural and Biological Sciences 4 7%
Immunology and Microbiology 4 7%
Biochemistry, Genetics and Molecular Biology 3 5%
Nursing and Health Professions 3 5%
Other 10 17%
Unknown 21 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2019.
All research outputs
#16,099,609
of 23,881,329 outputs
Outputs from BMC Pregnancy and Childbirth
#3,126
of 4,379 outputs
Outputs of similar age
#211,604
of 328,679 outputs
Outputs of similar age from BMC Pregnancy and Childbirth
#117
of 137 outputs
Altmetric has tracked 23,881,329 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,379 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.0. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 328,679 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 137 others from the same source and published within six weeks on either side of this one. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.