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The complete methylome of Helicobacter pylori UM032

Overview of attention for article published in BMC Genomics, June 2015
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  • Good Attention Score compared to outputs of the same age (68th percentile)
  • Good Attention Score compared to outputs of the same age and source (67th percentile)

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Title
The complete methylome of Helicobacter pylori UM032
Published in
BMC Genomics, June 2015
DOI 10.1186/s12864-015-1585-2
Pubmed ID
Authors

Woon Ching Lee, Brian P Anton, Susana Wang, Primo Baybayan, Siddarth Singh, Meredith Ashby, Eng Guan Chua, Chin Yen Tay, Fanny Thirriot, Mun Fai Loke, Khean Lee Goh, Barry J Marshall, Richard J Roberts, Jamuna Vadivelu

Abstract

The genome of the human gastric pathogen Helicobacter pylori encodes a large number of DNA methyltransferases (MTases), some of which are shared among many strains, and others of which are unique to a given strain. The MTases have potential roles in the survival of the bacterium. In this study, we sequenced a Malaysian H. pylori clinical strain, designated UM032, by using a combination of PacBio Single Molecule, Real-Time (SMRT) and Illumina MiSeq next generation sequencing platforms, and used the SMRT data to characterize the set of methylated bases (the methylome). The N4-methylcytosine and N6-methyladenine modifications detected at single-base resolution using SMRT technology revealed 17 methylated sequence motifs corresponding to one Type I and 16 Type II restriction-modification (R-M) systems. Previously unassigned methylation motifs were now assigned to their respective MTases-coding genes. Furthermore, one gene that appears to be inactive in the H. pylori UM032 genome during normal growth was characterized by cloning. Consistent with previously-studied H. pylori strains, we show that strain UM032 contains a relatively large number of R-M systems, including some MTase activities with novel specificities. Additional studies are underway to further elucidating the biological significance of the R-M systems in the physiology and pathogenesis of H. pylori.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 3%
Canada 1 2%
Unknown 57 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 30%
Researcher 14 23%
Student > Doctoral Student 5 8%
Student > Master 4 7%
Student > Bachelor 3 5%
Other 8 13%
Unknown 8 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 37%
Biochemistry, Genetics and Molecular Biology 18 30%
Medicine and Dentistry 3 5%
Immunology and Microbiology 2 3%
Computer Science 1 2%
Other 2 3%
Unknown 12 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2015.
All research outputs
#7,903,747
of 25,393,528 outputs
Outputs from BMC Genomics
#3,437
of 11,252 outputs
Outputs of similar age
#87,892
of 282,113 outputs
Outputs of similar age from BMC Genomics
#81
of 245 outputs
Altmetric has tracked 25,393,528 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 11,252 research outputs from this source. They receive a mean Attention Score of 4.8. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,113 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 245 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.