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Different maturation cocktails provide dendritic cells with different chemoattractive properties

Overview of attention for article published in Journal of Translational Medicine, June 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (71st percentile)

Mentioned by

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3 tweeters
patent
1 patent

Citations

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18 Dimensions

Readers on

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38 Mendeley
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Title
Different maturation cocktails provide dendritic cells with different chemoattractive properties
Published in
Journal of Translational Medicine, June 2015
DOI 10.1186/s12967-015-0528-7
Pubmed ID
Authors

Chiara Massa, Carolin Thomas, Ena Wang, Francesco Marincola, Barbara Seliger

Abstract

Dendritic cells (DC) are currently implemented as immunotherapeutic strategy for the treatment of tumor patients based on their central role in the immune system. Despite good results were obtained in vitro and in animal models, their clinical use has provided limited success suggesting the requirement to optimise the protocol for their production. A cDNA array was performed on FastDC obtained from the differentiation of human peripheral blood monocytes stimulated with the clinical gold standard or with two alternative maturation cocktails combining interferon (IFN)γ and ligands for different toll like receptors (TLR). A stronger modulation of the DC transcriptome with respect to immature DC was found in alternatively stimulated DC when compared to DC stimulated with the clinical gold standard. A major class of molecules differentially expressed using distinct DC stimulation protocols were chemokines. Validation of their differential expression pattern at the mRNA and protein level confirmed the secretion of inflammatory chemokines by the alternative DC. Functional analyses of the chemotactic properties of DC "wash out" supernatants highlighted the ability of alternative, but not of gold standard DC to efficiently recruit immune cells with a prevalence of monocytes. Effector cells belonging to the innate as well as adaptive immunity were also attracted and the interaction with alternative DC resulted in enhanced secretion of IFNγ and induction of cytotoxic activity. Using leukocytes from cancer patients, it was demonstrated that the monocyte-attracting activity targeted cells with an inflammatory phenotype characterised by high levels of HLA-DR expression. Despite other classes of immune modulatory genes differently expressed in the alternative DC require to be investigated and characterised regarding their functional consequences, the reduced maturation state and chemoattractive properties of the gold standard versus alternative DC clearly promote the necessity to change the clinically used maturation cocktail of DC in order to improve the outcome of patients treated with DC-based vaccines.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
Unknown 37 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 29%
Student > Master 10 26%
Student > Bachelor 7 18%
Student > Ph. D. Student 3 8%
Student > Doctoral Student 2 5%
Other 3 8%
Unknown 2 5%
Readers by discipline Count As %
Medicine and Dentistry 10 26%
Immunology and Microbiology 8 21%
Agricultural and Biological Sciences 7 18%
Biochemistry, Genetics and Molecular Biology 7 18%
Computer Science 1 3%
Other 2 5%
Unknown 3 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 December 2019.
All research outputs
#4,797,464
of 16,502,283 outputs
Outputs from Journal of Translational Medicine
#720
of 3,095 outputs
Outputs of similar age
#67,827
of 238,585 outputs
Outputs of similar age from Journal of Translational Medicine
#1
of 1 outputs
Altmetric has tracked 16,502,283 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 3,095 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,585 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them