Title |
Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community
|
---|---|
Published in |
Microbiome, May 2018
|
DOI | 10.1186/s40168-018-0475-7 |
Pubmed ID | |
Authors |
Efrem S. Lim, Cynthia Rodriguez, Lori R. Holtz |
Abstract |
Recent studies have conflicting data regarding the presence of intra-amniotic microbiota. Viral communities are increasingly recognized as important although overlooked components of the human microbiota. It is unknown if the developing fetus is exposed to a community of viruses (virome). Given the debate over the existence of an intra-amniotic microbial community and the importance of understanding how the infant gut is populated, we characterized the virome and bacterial microbiota of amniotic fluid from 24 uncomplicated term pregnancies using next-generation sequencing methods. Contrary to expectations, the bacterial microbiota of amniotic fluid was indistinguishable from contamination controls. Viral reads were sparse in the amniotic fluid, and we found no evidence of a core viral community across samples. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 14 | 21% |
United Kingdom | 5 | 7% |
Spain | 4 | 6% |
Australia | 4 | 6% |
Ireland | 3 | 4% |
Sweden | 2 | 3% |
Canada | 2 | 3% |
Singapore | 1 | 1% |
New Zealand | 1 | 1% |
Other | 5 | 7% |
Unknown | 27 | 40% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 36 | 53% |
Members of the public | 28 | 41% |
Practitioners (doctors, other healthcare professionals) | 3 | 4% |
Science communicators (journalists, bloggers, editors) | 1 | 1% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 149 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 26 | 17% |
Researcher | 25 | 17% |
Student > Master | 14 | 9% |
Student > Bachelor | 12 | 8% |
Student > Doctoral Student | 11 | 7% |
Other | 23 | 15% |
Unknown | 38 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 25 | 17% |
Immunology and Microbiology | 25 | 17% |
Biochemistry, Genetics and Molecular Biology | 20 | 13% |
Agricultural and Biological Sciences | 16 | 11% |
Engineering | 4 | 3% |
Other | 12 | 8% |
Unknown | 47 | 32% |