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Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells

Overview of attention for article published in Cellular & Molecular Biology Letters, March 2018
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Title
Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells
Published in
Cellular & Molecular Biology Letters, March 2018
DOI 10.1186/s11658-018-0080-6
Pubmed ID
Authors

Ghmkin Hassan, Mohammad Bahjat, Issam Kasem, Chadi Soukkarieh, Majd Aljamali

Abstract

Articular cartilage has a poor capacity for self-repair, and thus still presents a major challenge in orthopedics. Mesenchymal stem cells (MSCs) are multipotent stem cells with the potential to differentiate into chondrocytes in the presence of transforming growth factor beta (TGF-β). Platelet lysate (PL) contains a relatively large number of growth factors, including TGF-β, and has been shown to ameliorate cartilage repair. Here, we investigated the ability of PL to direct chondrogenic differentiation of MSCs along with other standard differentiation components in a pellet culture system. We isolated and expanded MSCs from human umbilical cords using a PL-supplemented medium and characterized the cells based on immunophenotype and potential for differentiation to adipocytes and osteocytes. We further cultured MSCs as pellets in a chondrogenic-differentiation medium supplemented with PL. After 21 days, the pellets were processed for histological analysis and stained with alician blue and acridine orange. The expression of SOX9 was investigated using RT-PCR. MSCs maintained their stemness characteristics in the PL-supplemented medium. However, the distribution of cells in the pellets cultured in the PL-supplemented chondrogenic differentiation medium had a greater similarity to cartilage tissue-derived chondrocytes than to the negative control. The intense alician blue staining indicated an increased production of mucopolysaccharides in the differentiated pellets, which also showed elevated expression of SOX9. Our data suggest that MSCs could be differentiated to chondrocytes in the presence of PL and absence of exogenous TGF-β. Further research needs to be conducted to understand the exact role and potential of PL in chondrogenic differentiation and chondrocyte regeneration.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 25%
Student > Ph. D. Student 5 21%
Student > Master 2 8%
Other 1 4%
Student > Doctoral Student 1 4%
Other 2 8%
Unknown 7 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 38%
Medicine and Dentistry 3 13%
Agricultural and Biological Sciences 2 8%
Materials Science 1 4%
Engineering 1 4%
Other 0 0%
Unknown 8 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2018.
All research outputs
#8,110,223
of 12,931,128 outputs
Outputs from Cellular & Molecular Biology Letters
#62
of 197 outputs
Outputs of similar age
#160,891
of 270,098 outputs
Outputs of similar age from Cellular & Molecular Biology Letters
#1
of 1 outputs
Altmetric has tracked 12,931,128 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 197 research outputs from this source. They receive a mean Attention Score of 2.2. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 270,098 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them