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No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology

Overview of attention for article published in Journal of Biological Engineering, June 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

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9 X users
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1 Facebook page

Citations

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7 Dimensions

Readers on

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32 Mendeley
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Title
No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology
Published in
Journal of Biological Engineering, June 2015
DOI 10.1186/s13036-015-0006-z
Pubmed ID
Authors

Afnan Azizi, Wilson Lam, Hilary Phenix, Lioudmila Tepliakova, Ian J Roney, Daniel Jedrysiak, Alex Power, Vaibhav Gupta, Nada Elnour, Martin Hanzel, Alexandra C Tzahristos, Shihab Sarwar, Mads Kærn

Abstract

The Registry of Standard Biological Parts imposes sequence constraints to enable DNA assembly using restriction enzymes. Alnahhas et al. (Journal of Biological Engineering 2014, 8:28) recently argued that these constraints should be revised because they impose an unnecessary burden on contributors that use homology-based assembly. To add to this debate, we tested four different homology-based methods, and found that students using these methods on their first attempt have a high probability of success. Because of their ease of use and high success rates, we believe that homology-based assembly is a best practice of Synthetic Biology, and recommend that the Registry implement the changes proposed by Alnahhas et al. to better support their use.

X Demographics

X Demographics

The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
China 1 3%
Unknown 30 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 25%
Student > Ph. D. Student 5 16%
Student > Bachelor 5 16%
Professor 3 9%
Other 2 6%
Other 7 22%
Unknown 2 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 41%
Biochemistry, Genetics and Molecular Biology 11 34%
Chemical Engineering 1 3%
Computer Science 1 3%
Medicine and Dentistry 1 3%
Other 2 6%
Unknown 3 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2015.
All research outputs
#5,503,689
of 22,813,792 outputs
Outputs from Journal of Biological Engineering
#85
of 260 outputs
Outputs of similar age
#63,497
of 264,937 outputs
Outputs of similar age from Journal of Biological Engineering
#2
of 6 outputs
Altmetric has tracked 22,813,792 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 260 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,937 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.