Title |
Rewriting the blueprint of life by synthetic genomics and genome engineering
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Published in |
Genome Biology, June 2015
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DOI | 10.1186/s13059-015-0689-y |
Pubmed ID | |
Authors |
Narayana Annaluru, Sivaprakash Ramalingam, Srinivasan Chandrasegaran |
Abstract |
Advances in DNA synthesis and assembly methods over the past decade have made it possible to construct genome-size fragments from oligonucleotides. Early work focused on synthesis of small viral genomes, followed by hierarchical synthesis of wild-type bacterial genomes and subsequently on transplantation of synthesized bacterial genomes into closely related recipient strains. More recently, a synthetic designer version of yeast Saccharomyces cerevisiae chromosome III has been generated, with numerous changes from the wild-type sequence without having an impact on cell fitness and phenotype, suggesting plasticity of the yeast genome. A project to generate the first synthetic yeast genome - the Sc2.0 Project - is currently underway. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 4 | 17% |
United States | 4 | 17% |
India | 2 | 9% |
Australia | 2 | 9% |
France | 1 | 4% |
Italy | 1 | 4% |
Unknown | 9 | 39% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 16 | 70% |
Scientists | 3 | 13% |
Practitioners (doctors, other healthcare professionals) | 3 | 13% |
Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | <1% |
Netherlands | 1 | <1% |
Chile | 1 | <1% |
Canada | 1 | <1% |
Peru | 1 | <1% |
Belgium | 1 | <1% |
Unknown | 118 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 32 | 26% |
Student > Ph. D. Student | 29 | 23% |
Student > Bachelor | 15 | 12% |
Student > Master | 11 | 9% |
Professor | 9 | 7% |
Other | 16 | 13% |
Unknown | 12 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 49 | 40% |
Biochemistry, Genetics and Molecular Biology | 34 | 27% |
Computer Science | 6 | 5% |
Engineering | 4 | 3% |
Medicine and Dentistry | 3 | 2% |
Other | 14 | 11% |
Unknown | 14 | 11% |