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Genome-wide identification and comprehensive analysis of microRNAs and phased small interfering RNAs in watermelon

Overview of attention for article published in BMC Genomics, May 2018
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Title
Genome-wide identification and comprehensive analysis of microRNAs and phased small interfering RNAs in watermelon
Published in
BMC Genomics, May 2018
DOI 10.1186/s12864-018-4457-8
Pubmed ID
Authors

Li Liu, Shuchao Ren, Junqiang Guo, Qingyi Wang, Xiaotuo Zhang, Peiran Liao, Shipeng Li, Ramanjulu Sunkar, Yun Zheng

Abstract

MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs involved in the post-transcriptional gene regulation and play a critical role in plant growth, development and stress responses. Watermelon (Citrullus lanatus L.) is one of the important agricultural crops worldwide. However, the watermelon miRNAs and phasiRNAs and their functions are not well explored. Here we carried out computational and experimental analysis of miRNAs and phased small interfering RNAs (phasiRNAs) in watermelon by analyzing 14 small RNA profiles from roots, leaves, androecium, petals, and fruits, and one published small RNA profile of mixed tissues. To identify the targets of miRNAs and phasiRNAs, we generated a degradome profile for watermelon leaf which is analyzed using the SeqTar algorithm. We identified 97 conserved pre-miRNAs, of which 58 have not been reported previously and 348 conserved mature miRNAs without precursors. We also found 9 novel pre-miRNAs encoding 18 mature miRNAs. One hundred and one 21 nucleotide (nt) PHAS loci, and two hundred and forty one 24 nt PHAS loci were also identified. We identified 127 conserved targets of the conserved miRNAs and TAS3-derived tasiRNAs by analyzing a degradome profile of watermelon leaf. The presented results provide a comprehensive view of small regulatory RNAs and their targets in watermelon.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 19%
Researcher 4 15%
Student > Doctoral Student 2 8%
Student > Postgraduate 2 8%
Student > Master 2 8%
Other 4 15%
Unknown 7 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 31%
Biochemistry, Genetics and Molecular Biology 6 23%
Computer Science 2 8%
Nursing and Health Professions 1 4%
Unknown 9 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2018.
All research outputs
#18,612,796
of 23,056,273 outputs
Outputs from BMC Genomics
#8,231
of 10,702 outputs
Outputs of similar age
#253,698
of 327,414 outputs
Outputs of similar age from BMC Genomics
#184
of 250 outputs
Altmetric has tracked 23,056,273 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,702 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
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