Title |
SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma
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Published in |
BMC Genomics, May 2018
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DOI | 10.1186/s12864-018-4746-2 |
Pubmed ID | |
Authors |
Antonio Jordán-Pla, Simei Yu, Johan Waldholm, Thomas Källman, Ann-Kristin Östlund Farrants, Neus Visa |
Abstract |
Brahma (BRM) is the only catalytic subunit of the SWI/SNF chromatin-remodeling complex of Drosophila melanogaster. The function of SWI/SNF in transcription has long been attributed to its ability to remodel nucleosomes, which requires the ATPase activity of BRM. However, recent studies have provided evidence for a non-catalytic function of BRM in the transcriptional regulation of a few specific genes. Here we have used RNA-seq and ChIP-seq to identify the BRM target genes in S2 cells, and we have used a catalytically inactive BRM mutant (K804R) that is unable to hydrolyze ATP to investigate the magnitude of the non-catalytic function of BRM in transcription regulation. We show that 49% of the BRM target genes in S2 cells are regulated through mechanisms that do not require BRM to have an ATPase activity. We also show that the catalytic and non-catalytic mechanisms of SWI/SNF regulation operate on two subsets of genes that differ in promoter architecture and are linked to different biological processes. This study shows that the non-catalytic role of SWI/SNF in transcription regulation is far more prevalent than previously anticipated and that the genes that are regulated by SWI/SNF through ATPase-dependent and ATPase-independent mechanisms have specialized roles in different cellular and developmental processes. |
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United States | 4 | 40% |
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Demographic breakdown
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Student > Bachelor | 7 | 18% |
Researcher | 5 | 13% |
Student > Doctoral Student | 4 | 10% |
Student > Master | 3 | 8% |
Other | 3 | 8% |
Unknown | 7 | 18% |
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Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
Physics and Astronomy | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Other | 0 | 0% |
Unknown | 7 | 18% |