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Molecular characterisation of the tick Rhipicephalus microplus in Malaysia: new insights into the cryptic diversity and distinct genetic assemblages throughout the world

Overview of attention for article published in Parasites & Vectors, June 2015
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Title
Molecular characterisation of the tick Rhipicephalus microplus in Malaysia: new insights into the cryptic diversity and distinct genetic assemblages throughout the world
Published in
Parasites & Vectors, June 2015
DOI 10.1186/s13071-015-0956-5
Pubmed ID
Authors

Van Lun Low, Sun Tee Tay, Kai Ling Kho, Fui Xian Koh, Tiong Kai Tan, Yvonne Ai Lian Lim, Bee Lee Ong, Chandrawathani Panchadcharam, Yusoff Norma-Rashid, Mohd Sofian-Azirun

Abstract

The morphotaxonomy of Rhipicephalus microplus complex has been challenged in the last few years and prompted many biologists to adopt a DNA-based method for distinguishing the members of this group. In the present study, we used a mitochondrial DNA analysis to characterise the genetic assemblages, population structure and dispersal pattern of R. microplus from Southeast Asia, the region where the species originated. A phylogeographic analysis inferred from the 16S rRNA and cytochrome oxidase subunit I (COI) genes was performed with five populations of R. microplus collected from cattle in Malaysia. Malaysian R. microplus sequences were compared with existing COI and 16S rRNA haplotypes reported globally in NCBI GenBank. A total of seven and 12 unique haplotypes were recovered by the 16S rRNA and COI genes, respectively. The concatenated sequences of both 16S rRNA and COI revealed 18 haplotypes. Haplotype network and phylogenetic analyses based on COI+16S rRNA sequences revealed four genetically divergent groups among Malaysian R. microplus. The significantly low genetic differentiation and high gene flow among Malaysian R. microplus populations supports the occurrence of genetic admixture. In a broader context, the 16S rRNA phylogenetic tree assigned all isolates of Malaysian R. microplus into the previously described African/Americas assemblage. However, the COI phylogenetic tree provides higher resolution of R. microplus with the identification of three main assemblages: clade A sensu Burger et al. (2014) comprises ticks from Southeast Asia, the Americas and China; clade B sensu Burger et al. (2014) is restricted to ticks that originated from China; and clade C sensu Low et al. (2015) is a new genetic assemblage discovered in this study comprising ticks from India and Malaysia. We conclude that the R. microplus complex consisting of at least five taxa: R. australis, R. annulatus, R. microplus clade A sensu Burger et al. (2014), R. microplus clade B sensu Burger et al. (2014) and the new taxon, R. microplus clade C sensu Low et al. (2015). The use of COI as the standard genetic marker in discerning the genetic assemblages of R. microplus from a broad range of biogeographical regions is proposed.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 109 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 17%
Student > Bachelor 14 13%
Student > Master 12 11%
Researcher 9 8%
Student > Postgraduate 8 7%
Other 28 26%
Unknown 20 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 37%
Veterinary Science and Veterinary Medicine 17 16%
Medicine and Dentistry 8 7%
Biochemistry, Genetics and Molecular Biology 8 7%
Immunology and Microbiology 4 4%
Other 3 3%
Unknown 29 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 June 2015.
All research outputs
#18,417,643
of 22,815,414 outputs
Outputs from Parasites & Vectors
#4,224
of 5,461 outputs
Outputs of similar age
#189,618
of 264,049 outputs
Outputs of similar age from Parasites & Vectors
#89
of 120 outputs
Altmetric has tracked 22,815,414 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
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