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Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells

Overview of attention for article published in BMC Biotechnology, June 2015
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  • Good Attention Score compared to outputs of the same age (74th percentile)

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3 tweeters
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1 patent

Citations

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3 Dimensions

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8 Mendeley
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Title
Secretion of Fc-amidated peptide fusion proteins by Chinese hamster ovary cells
Published in
BMC Biotechnology, June 2015
DOI 10.1186/s12896-015-0173-5
Pubmed ID
Authors

Kristina R. Carlson, Steven C. Pomerantz, Jiali Li, Omid Vafa, Michael Naso, William Strohl, Richard E. Mains, Betty A. Eipper

Abstract

The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the peptides would be useful. Both problems are potentially solved by producing peptides as fusions with the Fc region of human immunoglobulin. Glucagon-like peptide 1 (GLP1), peptide YY (PYY) and neuromedin U (NMU) were expressed and purified from stable CHO lines; since the α-amide group is essential for full biological potency of many peptides, Fc-fusion peptides were expressed in CHO lines stably expressing the α-amidating enzyme, peptidylglycine α-amidating monooxygenase (PAM: EC 1.14.17.3). Purified fusion proteins were analyzed intact and after HRV3C rhinovirus protease cleavage, at a site in the linker separating the Fc region from the peptide, by mass spectrometry and amide-specific immunoassays. The Fc fusions were expressed at 1-2.5 μg/mg cell protein and secreted at 5-20 % of cell content per hour, in a peptide-specific manner. CHO cells express measurable endogenous PAM activity, amidating 25 % of Fc-PYY and almost 90 % of Fc-GLP1. Expression of exogenous PAM increased the level of peptide amidation to 50 % of Fc-PYY and 95 % of Fc-NMU. The Fc-GLP1 fusions were 10,000-fold less active than synthetic GLP1 in a cell-receptor cyclic AMP-based assay, as expected since the amino terminal of GLP1 is essential for full biological activity. The Fc-PYY fusions were 100-fold less active than PYY-NH2 but 10-fold more active than non-amidated PYY-Gly. This type of approach can be used for the production of stabilized α-amidated peptides aimed at clinical trials.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 50%
Other 2 25%
Professor > Associate Professor 1 13%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 38%
Biochemistry, Genetics and Molecular Biology 2 25%
Pharmacology, Toxicology and Pharmaceutical Science 2 25%
Unknown 1 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 October 2019.
All research outputs
#4,196,123
of 16,110,746 outputs
Outputs from BMC Biotechnology
#265
of 827 outputs
Outputs of similar age
#58,397
of 234,899 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 16,110,746 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 827 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 234,899 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them