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Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species

Overview of attention for article published in Stem Cell Research & Therapy, July 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

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5 X users
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5 patents

Citations

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608 Dimensions

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537 Mendeley
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Title
Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species
Published in
Stem Cell Research & Therapy, July 2015
DOI 10.1186/s13287-015-0116-z
Pubmed ID
Authors

Serena Rubina Baglio, Koos Rooijers, Danijela Koppers-Lalic, Frederik J. Verweij, M Pérez Lanzón, Nicoletta Zini, Benno Naaijkens, Francesca Perut, Hans W. M. Niessen, Nicola Baldini, D. Michiel Pegtel

Abstract

Administration of mesenchymal stem cells (MSCs) represents a promising treatment option for patients suffering from immunological and degenerative disorders. Accumulating evidence indicates that the healing effects of MSCs are mainly related to unique paracrine properties, opening opportunities for secretome-based therapies. Apart from soluble factors, MSCs release functional small RNAs via extracellular vesicles (EVs) that seem to convey essential features of MSCs. Here we set out to characterize the full small RNAome of MSC-produced exosomes. We set up a protocol for isolating exosomes released by early passage adipose- (ASC) and bone marrow-MSCs (BMSC) and characterized them via electron microscopy, protein analysis and small RNA-sequencing. We developed a bioinformatics pipeline to define the exosome-enclosed RNA species and performed the first complete small RNA characterization of BMSCs and ASCs and their corresponding exosomes in biological replicates. Our analysis revealed that primary ASCs and BMSCs have highly similar small RNA expression profiles dominated by miRNAs and snoRNAs (together 64-71 %), of which 150-200 miRNAs are present at physiological levels. In contrast, the miRNA pool in MSC exosomes is only 2-5 % of the total small RNAome and is dominated by a minor subset of miRNAs. Nevertheless, the miRNAs in exosomes do not merely reflect the cellular content and a defined set of miRNAs are overrepresented in exosomes compared to the cell of origin. Moreover, multiple highly expressed miRNAs are precluded from exosomal sorting, consistent with the notion that these miRNAs are involved in functional repression of RNA targets. While ASC and BMSC exosomes are similar in RNA class distribution and composition, we observed striking differences in the sorting of evolutionary conserved tRNA species that seems associated with the differentiation status of MSCs, as defined by Sox2, POU5F1A/B and Nanog expression. We demonstrate that primary MSCs release small RNAs via exosomes, which are increasingly implicated in intercellular communications. tRNAs species, and in particular tRNA halves, are preferentially released and their specific sorting into exosomes is related to MSC tissue origin and stemness. These findings may help to understand how MSCs impact neighboring or distant cells with possible consequences for their therapeutic usage.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 537 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 <1%
Germany 1 <1%
United Kingdom 1 <1%
Iran, Islamic Republic of 1 <1%
Canada 1 <1%
Denmark 1 <1%
Poland 1 <1%
Unknown 529 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 93 17%
Researcher 79 15%
Student > Master 79 15%
Student > Bachelor 49 9%
Student > Doctoral Student 30 6%
Other 91 17%
Unknown 116 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 160 30%
Medicine and Dentistry 77 14%
Agricultural and Biological Sciences 76 14%
Immunology and Microbiology 17 3%
Engineering 16 3%
Other 53 10%
Unknown 138 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 May 2023.
All research outputs
#3,012,977
of 25,563,770 outputs
Outputs from Stem Cell Research & Therapy
#231
of 2,772 outputs
Outputs of similar age
#36,334
of 277,885 outputs
Outputs of similar age from Stem Cell Research & Therapy
#4
of 36 outputs
Altmetric has tracked 25,563,770 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,772 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,885 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.