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Genetical genomics of Populus leaf shape variation

Overview of attention for article published in BMC Plant Biology, June 2015
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Title
Genetical genomics of Populus leaf shape variation
Published in
BMC Plant Biology, June 2015
DOI 10.1186/s12870-015-0557-7
Pubmed ID
Authors

Derek R. Drost, Swati Puranik, Evandro Novaes, Carolina R.D.B. Novaes, Christopher Dervinis, Oliver Gailing, Matias Kirst

Abstract

Leaf morphology varies extensively among plant species and is under strong genetic control. Mutagenic screens in model systems have identified genes and established molecular mechanisms regulating leaf initiation, development, and shape. However, it is not known whether this diversity across plant species is related to naturally occurring variation at these genes. Quantitative trait locus (QTL) analysis has revealed a polygenic control for leaf shape variation in different species suggesting that loci discovered by mutagenesis may only explain part of the naturally occurring variation in leaf shape. Here we undertook a genetical genomics study in a poplar intersectional pseudo-backcross pedigree to identify genetic factors controlling leaf shape. The approach combined QTL discovery in a genetic linkage map anchored to the Populus trichocarpa reference genome sequence and transcriptome analysis. A major QTL for leaf lamina width and length:width ratio was identified in multiple experiments that confirmed its stability. A transcriptome analysis of expanding leaf tissue contrasted gene expression between individuals with alternative QTL alleles, and identified an ADP-ribosylation factor (ARF) GTPase (PtARF1) as a candidate gene for regulating leaf morphology in this pedigree. ARF GTPases are critical elements in the vesicular trafficking machinery. Disruption of the vesicular trafficking function of ARF by the pharmacological agent Brefeldin A (BFA) altered leaf lateral growth in the narrow-leaf P. trichocarpa suggesting a molecular mechanism of leaf shape determination. Inhibition of the vesicular trafficking processes by BFA interferes with cycling of PIN proteins and causes their accumulation in intercellular compartments abolishing polar localization and disrupting normal auxin flux with potential effects on leaf expansion. In other model systems, ARF proteins have been shown to control the localization of auxin efflux carriers, which function to establish auxin gradients and apical-basal cell polarity in developing plant organs. Our results support a model where PtARF1 transcript abundance changes the dynamics of endocytosis-mediated PIN localization in leaf cells, thus affecting lateral auxin flux and subsequently lamina leaf expansion. This suggests that evolution of differential cellular polarity plays a significant role in leaf morphological variation observed in subgenera of genus Populus.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Sweden 1 2%
Brazil 1 2%
Unknown 51 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 26%
Student > Ph. D. Student 13 24%
Professor 6 11%
Professor > Associate Professor 5 9%
Student > Postgraduate 4 7%
Other 7 13%
Unknown 5 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 35 65%
Biochemistry, Genetics and Molecular Biology 9 17%
Psychology 2 4%
Environmental Science 1 2%
Neuroscience 1 2%
Other 1 2%
Unknown 5 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2015.
All research outputs
#18,418,694
of 22,816,807 outputs
Outputs from BMC Plant Biology
#2,093
of 3,246 outputs
Outputs of similar age
#188,899
of 262,911 outputs
Outputs of similar age from BMC Plant Biology
#51
of 68 outputs
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