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Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH

Overview of attention for article published in Biomarker Research, July 2015
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  • Above-average Attention Score compared to outputs of the same age (51st percentile)

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2 tweeters

Citations

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14 Mendeley
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Title
Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH
Published in
Biomarker Research, July 2015
DOI 10.1186/s40364-015-0039-y
Pubmed ID
Authors

Valentina Rosso, Enrico Bracco, Roberto Pedrola, Sonia Carturan, Elisabetta Signorino, Jessica Petiti, Chiara Calabrese, Paolo Nicoli, Marco De Gobbi, Valentina Gaidano, Daniela Gallo, Stefano Ulisciani, Carmen Fava, Giovanna Rege-Cambrin, Francesco Frassoni, Giuseppe Saglio, Daniela Cilloni

Abstract

Mutations of the BCR-ABL1 fusion gene represent a well established cause of resistance to tyrosine kinase inhibitors. Among the different mutations identified T315I is of particular concern since it is not effectively targeted by the majority of Tyrosine Kinase Inhibitors so far available. We developed a novel assay based on peptide nucleic acid (PNA) technology coupled to immunofluorescence microscopy (PNA-FISH) for the specific detection at a single cell level of BCR-ABL (T315I) mutation thus improving both, diagnostic resolution and the study of clonal prevalence. Furthermore we developed an additional method based on PNA directed PCR-clamping for the fast and easy detection of the mutation. The PNA directed PCR clamping allows to detect an amount of mutated template as low as 0.5 %. This method is highly sensitive, specific and cheap and could be applied even in laboratory not equipped for more sophisticated analysis. Furthermore, the PNA FISH method allows to identify a small amount of progenitor cells still present after therapy with specific inhibitors. We present here two different methods based on PNA for the detection of T315I useful for different purposes. PNA-FISH can be used to study clonal evolution. In addition, this method could help in the study of compound mutations being able to identify two different mutations in a single cell. PNA directed PCR clamping although not superior to sequencing can be applied worldwide even in laboratory not equipped to search for mutations.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 7%
Pakistan 1 7%
Unknown 12 86%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 2 14%
Researcher 2 14%
Student > Ph. D. Student 2 14%
Student > Bachelor 1 7%
Student > Master 1 7%
Other 2 14%
Unknown 4 29%
Readers by discipline Count As %
Medicine and Dentistry 4 29%
Agricultural and Biological Sciences 3 21%
Chemistry 1 7%
Materials Science 1 7%
Engineering 1 7%
Other 0 0%
Unknown 4 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2015.
All research outputs
#2,457,888
of 5,325,534 outputs
Outputs from Biomarker Research
#8
of 40 outputs
Outputs of similar age
#84,573
of 185,955 outputs
Outputs of similar age from Biomarker Research
#2
of 2 outputs
Altmetric has tracked 5,325,534 research outputs across all sources so far. This one has received more attention than most of these and is in the 51st percentile.
So far Altmetric has tracked 40 research outputs from this source. They receive a mean Attention Score of 1.6. This one scored the same or higher as 32 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 185,955 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.