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Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH

Overview of attention for article published in Biomarker Research, July 2015
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Title
Detection of BCR-ABL T315I mutation by peptide nucleic acid directed PCR clamping and by peptide nucleic acid FISH
Published in
Biomarker Research, July 2015
DOI 10.1186/s40364-015-0039-y
Pubmed ID
Authors

Valentina Rosso, Enrico Bracco, Roberto Pedrola, Sonia Carturan, Elisabetta Signorino, Jessica Petiti, Chiara Calabrese, Paolo Nicoli, Marco De Gobbi, Valentina Gaidano, Daniela Gallo, Stefano Ulisciani, Carmen Fava, Giovanna Rege-Cambrin, Francesco Frassoni, Giuseppe Saglio, Daniela Cilloni

Abstract

Mutations of the BCR-ABL1 fusion gene represent a well established cause of resistance to tyrosine kinase inhibitors. Among the different mutations identified T315I is of particular concern since it is not effectively targeted by the majority of Tyrosine Kinase Inhibitors so far available. We developed a novel assay based on peptide nucleic acid (PNA) technology coupled to immunofluorescence microscopy (PNA-FISH) for the specific detection at a single cell level of BCR-ABL (T315I) mutation thus improving both, diagnostic resolution and the study of clonal prevalence. Furthermore we developed an additional method based on PNA directed PCR-clamping for the fast and easy detection of the mutation. The PNA directed PCR clamping allows to detect an amount of mutated template as low as 0.5 %. This method is highly sensitive, specific and cheap and could be applied even in laboratory not equipped for more sophisticated analysis. Furthermore, the PNA FISH method allows to identify a small amount of progenitor cells still present after therapy with specific inhibitors. We present here two different methods based on PNA for the detection of T315I useful for different purposes. PNA-FISH can be used to study clonal evolution. In addition, this method could help in the study of compound mutations being able to identify two different mutations in a single cell. PNA directed PCR clamping although not superior to sequencing can be applied worldwide even in laboratory not equipped to search for mutations.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 6%
Pakistan 1 6%
Unknown 16 89%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 2 11%
Researcher 2 11%
Student > Ph. D. Student 2 11%
Other 1 6%
Student > Doctoral Student 1 6%
Other 3 17%
Unknown 7 39%
Readers by discipline Count As %
Medicine and Dentistry 5 28%
Agricultural and Biological Sciences 3 17%
Chemistry 1 6%
Materials Science 1 6%
Engineering 1 6%
Other 0 0%
Unknown 7 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2015.
All research outputs
#14,231,577
of 22,816,807 outputs
Outputs from Biomarker Research
#131
of 312 outputs
Outputs of similar age
#135,284
of 262,956 outputs
Outputs of similar age from Biomarker Research
#2
of 6 outputs
Altmetric has tracked 22,816,807 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 312 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 53% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,956 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.