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Differential replication properties among H9N2 avian influenza viruses of Eurasian origin

Overview of attention for article published in Veterinary Research, July 2015
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  • Above-average Attention Score compared to outputs of the same age (55th percentile)

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3 tweeters
1 Facebook page


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40 Mendeley
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Differential replication properties among H9N2 avian influenza viruses of Eurasian origin
Published in
Veterinary Research, July 2015
DOI 10.1186/s13567-015-0198-8
Pubmed ID

Rokshana Parvin, Awad A. Shehata, Kristin Heenemann, Malgorzata Gac, Antje Rueckner, Mohammad Y. Halami, Thomas W. Vahlenkamp


Avian influenza H9N2 viruses have become panzootic in Eurasia causing respiratory manifestations, great economic losses and occasionally being transmitted to humans. To evaluate the replication properties and compare the different virus quantification methods, four Eurasian H9N2 viruses from different geographical origins were propagated in embryonated chicken egg (ECE) and Madin-Darby canine kidney epithelial cell systems. The ECE-grown and cell culture-grown viruses were monitored for replication kinetics based on tissue culture infectious dose (TCID50), Hemagglutination (HA) test and quantitative real time RT-PCR (qRT-PCR). The cellular morphology was analyzed using immunofluorescence (IF) and cellular ELISA was used to screen the sensitivity of the viruses to amantadine. The Eurasian wild type-H9N2 virus produced lower titers compared to the three G1-H9N2 viruses at respective time points. Detectable titers were observed earliest at 16 h post inoculation (hpi), significant morphological changes on cells were first observed at 32 hpi. Few nucleotide and amino acid substitutions were noticed in the HA, NA and NS gene sequences but none of them are related to the known conserved region that can alter pathogenesis or virulence following a single passage in cell culture. All studied H9N2 viruses were sensitive to amantadine. The G1-H9N2 viruses have higher replication capabilities compared to the European wild bird-H9N2 probably due to their specific genetic constitutions which is prerequisite for a successful vaccine candidate. Both the ECE and MDCK cell system allowed efficient replication but the ECE system is considered as the better cultivation system for H9N2 viruses in order to get maximum amounts of virus within a short time period.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 3%
Unknown 39 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 25%
Researcher 8 20%
Student > Master 6 15%
Other 4 10%
Student > Postgraduate 2 5%
Other 3 8%
Unknown 7 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 25%
Immunology and Microbiology 6 15%
Biochemistry, Genetics and Molecular Biology 5 13%
Veterinary Science and Veterinary Medicine 4 10%
Medicine and Dentistry 4 10%
Other 1 3%
Unknown 10 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2015.
All research outputs
of 14,123,042 outputs
Outputs from Veterinary Research
of 881 outputs
Outputs of similar age
of 231,844 outputs
Outputs of similar age from Veterinary Research
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Altmetric has tracked 14,123,042 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 881 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,844 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them