Title |
Acquired transmissibility of sheep-passaged L-type bovine spongiform encephalopathy prion to wild-type mice
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Published in |
Veterinary Research, July 2015
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DOI | 10.1186/s13567-015-0211-2 |
Pubmed ID | |
Authors |
Hiroyuki Okada, Kentaro Masujin, Kohtaro Miyazawa, Takashi Yokoyama |
Abstract |
L-type bovine spongiform encephalopathy (L-BSE) is an atypical form of BSE that is transmissible to cattle and several lines of prion protein (PrP) transgenic mice, but not to wild-type mice. In this study, we examined the transmissibility of sheep-passaged L-BSE prions to wild-type mice. Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE. The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice. The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate. The data indicate that sheep-passaged L-BSE has an altered host range and acquired transmissibility to wild-type mice. |
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Members of the public | 2 | 100% |
Mendeley readers
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Unknown | 16 | 100% |
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Researcher | 6 | 38% |
Student > Ph. D. Student | 3 | 19% |
Student > Doctoral Student | 1 | 6% |
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Other | 1 | 6% |
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