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Proteomic analysis of Artemisia annua – towards elucidating the biosynthetic pathways of the antimalarial pro-drug artemisinin

Overview of attention for article published in BMC Plant Biology, July 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (68th percentile)

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4 tweeters
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1 Facebook page

Citations

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35 Dimensions

Readers on

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51 Mendeley
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Title
Proteomic analysis of Artemisia annua – towards elucidating the biosynthetic pathways of the antimalarial pro-drug artemisinin
Published in
BMC Plant Biology, July 2015
DOI 10.1186/s12870-015-0565-7
Pubmed ID
Authors

Laura Bryant, Brian Flatley, Chhaya Patole, Geoffrey D. Brown, Rainer Cramer

Abstract

MS-based proteomics was applied to the analysis of the medicinal plant Artemisia annua, exploiting a recently published contig sequence database (Graham et al. (2010) Science 327, 328-331) and other genomic and proteomic sequence databases for comparison. A. annua is the predominant natural source of artemisinin, the precursor for artemisinin-based combination therapies (ACTs), which are the WHO-recommended treatment for P. falciparum malaria. The comparison of various databases containing A. annua sequences (NCBInr/viridiplantae, UniProt/viridiplantae, UniProt/A. annua, an A. annua trichome Trinity contig database, the above contig database and another A. annua EST database) revealed significant differences in respect of their suitability for proteomic analysis, showing that an organism-specific database that has undergone extensive curation, leading to longer contig sequences, can greatly increase the number of true positive protein identifications, while reducing the number of false positives. Compared to previously published data an order-of-magnitude more proteins have been identified from trichome-enriched A. annua samples, including proteins which are known to be involved in the biosynthesis of artemisinin, as well as other highly abundant proteins, which suggest additional enzymatic processes occurring within the trichomes that are important for the biosynthesis of artemisinin. The newly gained information allows for the possibility of an enzymatic pathway, utilizing peroxidases, for the less well understood final stages of artemisinin's biosynthesis, as an alternative to the known non-enzymatic in vitro conversion of dihydroartemisinic acid to artemisinin. Data are available via ProteomeXchange with identifier PXD000703.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
Unknown 50 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 24%
Student > Bachelor 6 12%
Researcher 5 10%
Student > Doctoral Student 4 8%
Other 4 8%
Other 12 24%
Unknown 8 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 35%
Chemistry 8 16%
Biochemistry, Genetics and Molecular Biology 6 12%
Pharmacology, Toxicology and Pharmaceutical Science 4 8%
Chemical Engineering 1 2%
Other 5 10%
Unknown 9 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2015.
All research outputs
#4,968,513
of 15,880,595 outputs
Outputs from BMC Plant Biology
#465
of 2,301 outputs
Outputs of similar age
#73,192
of 232,892 outputs
Outputs of similar age from BMC Plant Biology
#1
of 1 outputs
Altmetric has tracked 15,880,595 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 2,301 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 232,892 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them