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GSK-3β inhibits autophagy and enhances radiosensitivity in non-small cell lung cancer

Overview of attention for article published in Diagnostic Pathology, May 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (51st percentile)

Mentioned by

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4 tweeters

Citations

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16 Dimensions

Readers on

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25 Mendeley
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Title
GSK-3β inhibits autophagy and enhances radiosensitivity in non-small cell lung cancer
Published in
Diagnostic Pathology, May 2018
DOI 10.1186/s13000-018-0708-x
Pubmed ID
Authors

Jialin Ren, Tingting Liu, Yang Han, Qiongzi Wang, Yanzhi Chen, Guang Li, Lihong Jiang

Abstract

Radiotherapy is one of the most common and effective treatment methods for cancer, and improving the radiosensitivity of tumor tissues during the treatment process is vital. We report the mechanisms of glycogen synthase kinase 3 (GSK-3) β-regulated autophagy and the effects of autophagy on radiosensitivity in non-small cell lung cancer (NSCLC). Immunohistochemical staining was performed to determine GSK-3β tissue expression in 89 NSCLC patients with follow-up data and the expression status of GSK-3β and autophagy in NSCLC tissues after X-ray radiotherapy. Western blots were used to quantitate changes in autophagy-related protein expression after A549 cells were treated with GSK-3β inhibitors and after H460 cells were transfected with GSK-3β mutants with different activities and X-ray irradiated. Clonogenic assays were used to measure the effect of autophagy on cellular proliferation. GSK-3β expression positively correlated with NSCLC differentiation (P < 0.05), and GSK-3β negativity was associated with a better prognosis in 89 NSCLC patients. After X-ray irradiation, the expression levels of GSK-3β and p62 were decreased in NSCLC tissues, and the expression levels of the autophagy-related protein LC3 were increased. A549 and H460 cells were selected as representative GSK-3β-high and GSK-3β-low expression cell lines. After transfecting H460 cells with different GSK-3β mutants [wild type GSK-3β (GSK-3β-WT), constitutively active GSK-3β (GSK-3β-S9A), and catalytically inactive GSK-3β (GSK-3β-K85R)] and subjecting these cells to X-ray irradiation, AMPK and LC3 expression levels decreased, and p62 expression levels increased. These effects were particularly significant for the GSK-3β-S9A mutant. In A549 cells, after GSK-3β inhibition and X-ray irradiation, AMPK and LC3 protein expression levels increased. Moreover, when autophagy was inhibited, cell proliferation decreased. Our studies revealed that GSK-3β expression is associated with NSCLC differentiation, and patients with GSK-3β-negative tumors had a better prognosis. X-ray irradiation inhibited GSK-3β expression and promoted autophagy. Therefore, GSK-3β inhibits autophagy and enhances the radiosensitivity of NSCLC cells.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 28%
Student > Master 4 16%
Student > Doctoral Student 2 8%
Student > Ph. D. Student 1 4%
Lecturer > Senior Lecturer 1 4%
Other 2 8%
Unknown 8 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 32%
Medicine and Dentistry 4 16%
Pharmacology, Toxicology and Pharmaceutical Science 2 8%
Chemistry 1 4%
Unknown 10 40%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2020.
All research outputs
#10,945,230
of 19,140,651 outputs
Outputs from Diagnostic Pathology
#285
of 1,008 outputs
Outputs of similar age
#141,452
of 293,710 outputs
Outputs of similar age from Diagnostic Pathology
#1
of 1 outputs
Altmetric has tracked 19,140,651 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,008 research outputs from this source. They receive a mean Attention Score of 2.5. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 293,710 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them