Title |
A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants
|
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Published in |
Plant Methods, May 2018
|
DOI | 10.1186/s13007-018-0305-8 |
Pubmed ID | |
Authors |
Jinggong Guo, Kun Li, Lifeng Jin, Rui Xu, Kaiting Miao, Fengbo Yang, Chaoya Qi, Lin Zhang, Jose R. Botella, Ran Wang, Yuchen Miao |
Abstract |
The CRISPR/Cas9 system is being used for genome editing purposes by many research groups in multiple plant species. Traditional sequencing methods to identify homozygous mutants are time-consuming, laborious and expensive. We have developed a method to screen CRISPR/Cas9-induced mutants through Mutation Sites Based Specific Primers Polymerase Chain Reaction (MSBSP-PCR). The MSBSP-PCR method was successfully used to identify homozygous/biallelic mutants in Nicotiana tabacum and Arabidopsis thaliana, and we speculate that it can be used for the identification of CRISPR/Cas9-induced mutants in other plant species. Compared to traditional sequencing methods, MSBSP-PCR is simpler, faster and cheaper. The MSBSP-PCR method is simple to implement and can save time and cost in the screening of CRISPR/Cas9-induced homozygous/biallelic mutants. |
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Geographical breakdown
Country | Count | As % |
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Canada | 2 | 29% |
United Kingdom | 2 | 29% |
Unknown | 3 | 43% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 71% |
Scientists | 1 | 14% |
Science communicators (journalists, bloggers, editors) | 1 | 14% |
Mendeley readers
Geographical breakdown
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Unknown | 111 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 20 | 18% |
Student > Master | 18 | 16% |
Researcher | 17 | 15% |
Student > Bachelor | 11 | 10% |
Other | 5 | 5% |
Other | 16 | 14% |
Unknown | 24 | 22% |
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Biochemistry, Genetics and Molecular Biology | 29 | 26% |
Environmental Science | 1 | <1% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | <1% |
Arts and Humanities | 1 | <1% |
Other | 4 | 4% |
Unknown | 29 | 26% |