↓ Skip to main content

Scrutinizing the immune defence inventory of Camponotus floridanus applying total transcriptome sequencing

Overview of attention for article published in BMC Genomics, July 2015
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

twitter
12 tweeters

Citations

dimensions_citation
28 Dimensions

Readers on

mendeley
64 Mendeley
citeulike
3 CiteULike
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Scrutinizing the immune defence inventory of Camponotus floridanus applying total transcriptome sequencing
Published in
BMC Genomics, July 2015
DOI 10.1186/s12864-015-1748-1
Pubmed ID
Authors

Shishir K. Gupta, Maria Kupper, Carolin Ratzka, Heike Feldhaar, Andreas Vilcinskas, Roy Gross, Thomas Dandekar, Frank Förster

Abstract

Defence mechanisms of organisms are shaped by their lifestyle, environment and pathogen pressure. Carpenter ants are social insects which live in huge colonies comprising genetically closely related individuals in high densities within nests. This lifestyle potentially facilitates the rapid spread of pathogens between individuals. In concert with their innate immune system, social insects may apply external immune defences to manipulate the microbial community among individuals and within nests. Additionally, carpenter ants carry a mutualistic intracellular and obligate endosymbiotic bacterium, possibly maintained and regulated by the innate immune system. Thus, different selective forces could shape internal immune defences of Camponotus floridanus. The immune gene repertoire of C. floridanus was investigated by re-evaluating its genome sequence combined with a full transcriptome analysis of immune challenged and control animals using Illumina sequencing. The genome was re-annotated by mapping transcriptome reads and masking repeats. A total of 978 protein sequences were characterised further by annotating functional domains, leading to a change in their original annotation regarding function and domain composition in about 8 % of all proteins. Based on homology analysis with key components of major immune pathways of insects, the C. floridanus immune-related genes were compared to those of Drosophila melanogaster, Apis mellifera, and other hymenoptera. This analysis revealed that overall the immune system of carpenter ants comprises many components found in these insects. In addition, several C. floridanus specific genes of yet unknown functions but which are strongly induced after immune challenge were discovered. In contrast to solitary insects like Drosophila or the hymenopteran Nasonia vitripennis, the number of genes encoding pattern recognition receptors specific for bacterial peptidoglycan (PGN) and a variety of known antimicrobial peptide (AMP) genes is lower in C. floridanus. The comparative analysis of gene expression post immune-challenge in different developmental stages of C. floridanus suggests a stronger induction of immune gene expression in larvae in comparison to adults. The comparison of the immune system of C. floridanus with that of other insects revealed the presence of a broad immune repertoire. However, the relatively low number of PGN recognition proteins and AMPs, the identification of Camponotus specific putative immune genes, and stage specific differences in immune gene regulation reflects Camponotus specific evolution including adaptations to its lifestyle.

Twitter Demographics

The data shown below were collected from the profiles of 12 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 3%
Austria 2 3%
Switzerland 1 2%
Germany 1 2%
Unknown 58 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 30%
Researcher 17 27%
Student > Master 7 11%
Other 3 5%
Professor 3 5%
Other 6 9%
Unknown 9 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 35 55%
Biochemistry, Genetics and Molecular Biology 15 23%
Immunology and Microbiology 2 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Computer Science 1 2%
Other 2 3%
Unknown 8 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 March 2016.
All research outputs
#954,247
of 7,435,912 outputs
Outputs from BMC Genomics
#676
of 5,446 outputs
Outputs of similar age
#38,801
of 226,977 outputs
Outputs of similar age from BMC Genomics
#31
of 273 outputs
Altmetric has tracked 7,435,912 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,446 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 226,977 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 273 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.