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Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish

Overview of attention for article published in BMC Genomics, July 2015
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  • Above-average Attention Score compared to outputs of the same age (60th percentile)

Mentioned by

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7 tweeters
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1 Facebook page

Citations

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22 Dimensions

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69 Mendeley
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Title
Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish
Published in
BMC Genomics, July 2015
DOI 10.1186/s12864-015-1740-9
Pubmed ID
Authors

Shuxin Yang, Rubén Marín-Juez, Annemarie H. Meijer, Herman P. Spaink

Abstract

Although the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole organisms. To characterize this in detail, we have performed RNAseq analysis of responses of zebrafish embryos to injection of PAMPs in the caudal vein at one hour after exposure. We have compared two ligands that in mammals have been shown to specifically activate the TLR2 and TLR5 receptors: Pam3CSK4 and flagellin, respectively. We identified a group of 80 common genes that respond with high stringency selection to stimulations with both PAMPs, which included several well-known immune marker genes such as il1b and tnfa. Surprisingly, we also identified sets of 48 and 42 genes that specifically respond to either Pam3CSK4 or flagellin, respectively, after a comparative filtering approach. Remarkably, in the Pam3CSK4 specific set, there was a set of transcription factors with more than 2 fold-change, as confirmed by qPCR analyses, including cebpb, fosb, nr4a1 and egr3. We also showed that the regulation of the Pam3CSK4 and flagellin specifically responding sets is inhibited by knockdown of tlr2 or tlr5, respectively. Our studies show that Pam3CSK4 and flagellin can stimulate the Tlr2 and Tlr5 signaling pathways leading to common and specific responses in the zebrafish embryo system.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 1%
United States 1 1%
Unknown 67 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 23%
Student > Bachelor 12 17%
Researcher 11 16%
Student > Master 9 13%
Student > Doctoral Student 4 6%
Other 12 17%
Unknown 5 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 24 35%
Immunology and Microbiology 14 20%
Biochemistry, Genetics and Molecular Biology 9 13%
Medicine and Dentistry 5 7%
Pharmacology, Toxicology and Pharmaceutical Science 5 7%
Other 4 6%
Unknown 8 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 March 2016.
All research outputs
#8,820,314
of 16,638,522 outputs
Outputs from BMC Genomics
#3,751
of 9,107 outputs
Outputs of similar age
#91,980
of 237,228 outputs
Outputs of similar age from BMC Genomics
#1
of 1 outputs
Altmetric has tracked 16,638,522 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,107 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 237,228 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them