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Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish

Overview of attention for article published in BMC Genomics, July 2015
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  • Above-average Attention Score compared to outputs of the same age (55th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

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Title
Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish
Published in
BMC Genomics, July 2015
DOI 10.1186/s12864-015-1740-9
Pubmed ID
Authors

Shuxin Yang, Rubén Marín-Juez, Annemarie H. Meijer, Herman P. Spaink

Abstract

Although the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole organisms. To characterize this in detail, we have performed RNAseq analysis of responses of zebrafish embryos to injection of PAMPs in the caudal vein at one hour after exposure. We have compared two ligands that in mammals have been shown to specifically activate the TLR2 and TLR5 receptors: Pam3CSK4 and flagellin, respectively. We identified a group of 80 common genes that respond with high stringency selection to stimulations with both PAMPs, which included several well-known immune marker genes such as il1b and tnfa. Surprisingly, we also identified sets of 48 and 42 genes that specifically respond to either Pam3CSK4 or flagellin, respectively, after a comparative filtering approach. Remarkably, in the Pam3CSK4 specific set, there was a set of transcription factors with more than 2 fold-change, as confirmed by qPCR analyses, including cebpb, fosb, nr4a1 and egr3. We also showed that the regulation of the Pam3CSK4 and flagellin specifically responding sets is inhibited by knockdown of tlr2 or tlr5, respectively. Our studies show that Pam3CSK4 and flagellin can stimulate the Tlr2 and Tlr5 signaling pathways leading to common and specific responses in the zebrafish embryo system.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 1%
United States 1 1%
Unknown 75 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 22%
Student > Bachelor 14 18%
Researcher 11 14%
Student > Master 10 13%
Student > Doctoral Student 5 6%
Other 12 16%
Unknown 8 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 32%
Immunology and Microbiology 14 18%
Biochemistry, Genetics and Molecular Biology 12 16%
Pharmacology, Toxicology and Pharmaceutical Science 6 8%
Medicine and Dentistry 5 6%
Other 4 5%
Unknown 11 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 March 2016.
All research outputs
#14,026,250
of 24,792,414 outputs
Outputs from BMC Genomics
#4,679
of 11,067 outputs
Outputs of similar age
#118,893
of 268,622 outputs
Outputs of similar age from BMC Genomics
#126
of 265 outputs
Altmetric has tracked 24,792,414 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,067 research outputs from this source. They receive a mean Attention Score of 4.8. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,622 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 265 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.