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Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovisin european wild boar

Overview of attention for article published in BMC Veterinary Research, November 2008
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (60th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

policy
1 policy source

Citations

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40 Dimensions

Readers on

mendeley
60 Mendeley
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Title
Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovisin european wild boar
Published in
BMC Veterinary Research, November 2008
DOI 10.1186/1746-6148-4-43
Pubmed ID
Authors

Olaia Aurtenetxe, Marta Barral, Joaquín Vicente, José de la Fuente, Christian Gortázar, Ramón A Juste

Abstract

Bovine tuberculosis (bTB) remains a significant problem in some parts of Spain largely because of contacts between cattle and wildlife reservoirs in extensive grazing systems. European Wild boar (Sus scrofa) is one of the species involved in the transmission of the disease to other species. Fast and simple detection methods would be critical for assessing infection prevalence, study the mechanisms of pathogen transmission and monitoring the effects of TB control measures. An enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Mycobacterium bovis in wild boar serum was developed and validated on 185 sera from TB positive and negative wild boar. Based on antigen inoculation of captive animals as well as tuberculosis compatible lesions, culture results and molecular analysis of hunted individuals, animals were allocated into two groups: tuberculosis positive group and tuberculosis negative group. After optimization of the positive to negative ratio using different combinations of serum dilutions and conjugate concentrations, the test yielded a sensitivity of 72.60% and a specificity of 96.43% for the best cut-off. Although some negative group animals showed an ELISA positive reaction (< 3%), this assay showed a high potential for accurate diagnosis of TB in wild boar, as its large dynamic range supported a good discriminatory power and a satisfactory balance between sensitivity and specificity.

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 2%
Spain 1 2%
South Africa 1 2%
Unknown 57 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 23%
Researcher 13 22%
Student > Master 9 15%
Student > Bachelor 4 7%
Student > Doctoral Student 4 7%
Other 11 18%
Unknown 5 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 27 45%
Veterinary Science and Veterinary Medicine 11 18%
Biochemistry, Genetics and Molecular Biology 3 5%
Environmental Science 3 5%
Immunology and Microbiology 3 5%
Other 5 8%
Unknown 8 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2009.
All research outputs
#6,095,704
of 18,787,703 outputs
Outputs from BMC Veterinary Research
#512
of 2,630 outputs
Outputs of similar age
#80,198
of 243,237 outputs
Outputs of similar age from BMC Veterinary Research
#2
of 11 outputs
Altmetric has tracked 18,787,703 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,630 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,237 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.