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Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation

Overview of attention for article published in BMC Genomics, June 2018
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Title
Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation
Published in
BMC Genomics, June 2018
DOI 10.1186/s12864-018-4826-3
Pubmed ID
Authors

Dessie Salilew-Wondim, Mohammed Saeed-Zidane, Michael Hoelker, Samuel Gebremedhn, Mikhaël Poirier, Hari Om Pandey, Ernst Tholen, Christiane Neuhoff, Eva Held, Urban Besenfelder, Vita Havlicek, Franca Rings, Eric Fournier, Dominic Gagné, Marc-André Sirard, Claude Robert, Ahmed Gad, Karl Schellander, Dawit Tesfaye

Abstract

Aberrant DNA methylation patterns of genes required for development are common in in vitro produced embryos. In this regard, we previously identified altered DNA methylation patterns of in vivo developed blastocysts from embryos which spent different stages of development in vitro, indicating carryover effects of suboptimal culture conditions on epigenetic signatures of preimplantation embryos. However, epigenetic responses of in vivo originated embryos to suboptimal culture conditions are not fully understood. Therefore, here we investigated DNA methylation patterns of in vivo derived bovine embryos subjected to in vitro culture condition before, during or after major embryonic genome activation (EGA). For this, in vivo produced 2-, 8- and 16-cell stage embryos were cultured in vitro until the blastocyst stage and blastocysts were used for genome-wide DNA methylation analysis. The 2- and 8-cell flushed embryo groups showed lower blastocyst rates compared to the 16-cell flush group. This was further accompanied by increased numbers of differentially methylated genomic regions (DMRs) in blastocysts of the 2- and 8-cell flush groups compared to the complete in vivo control ones. Moreover, 1623 genomic loci including imprinted genes were hypermethylated in blastocyst of 2-, 8- and 16-cell flushed groups, indicating the presence of genomic regions which are sensitive to the in vitro culture at any stage of embryonic development. Furthermore, hypermethylated genomic loci outnumbered hypomethylated ones in blastocysts of 2- and 16-cell flushed embryo groups, but the opposite occurred in the 8-cell group. Moreover, DMRs which were unique to blastocysts of the 2-cell flushed group and inversely correlated with corresponding mRNA expression levels were involved in plasma membrane lactate transport, amino acid transport and phosphorus metabolic processes, whereas DMRs which were specific to the 8-cell group and inversely correlated with corresponding mRNA expression levels were involved in several biological processes including regulation of fatty acids and steroid biosynthesis processes. In vivo embryos subjected to in vitro culture before and during major embryonic genome activation (EGA) are prone to changes in DNA methylation marks and exposure of in vivo embryos to in vitro culture during the time of EGA increased hypomethylated genomic loci in blastocysts.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 41 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 20%
Student > Master 6 15%
Student > Bachelor 4 10%
Student > Doctoral Student 3 7%
Professor 3 7%
Other 8 20%
Unknown 9 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 39%
Biochemistry, Genetics and Molecular Biology 4 10%
Medicine and Dentistry 4 10%
Veterinary Science and Veterinary Medicine 3 7%
Nursing and Health Professions 1 2%
Other 0 0%
Unknown 13 32%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2018.
All research outputs
#14,144,180
of 16,036,905 outputs
Outputs from BMC Genomics
#7,707
of 8,906 outputs
Outputs of similar age
#243,048
of 282,165 outputs
Outputs of similar age from BMC Genomics
#17
of 21 outputs
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