Title |
Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions
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Published in |
Biotechnology for Biofuels and Bioproducts, July 2015
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DOI | 10.1186/s13068-015-0286-z |
Pubmed ID | |
Authors |
Magdalena Rakicka, Zbigniew Lazar, Thierry Dulermo, Patrick Fickers, Jean Marc Nicaud |
Abstract |
Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculum densities was evaluated in fed-batch cultures. The greatest lipid content, 31% of CDW, was obtained using a low-density inoculum, a constant agitation rate of 800 rpm, and an oxygenation rate of 1.5 L/min. When the strain was cultured for 450 h in a chemostat containing a nitrogen-limited medium (dilution rate of 0.01 h(-1); 250 g/L crude glycerol), volumetric lipid productivity was 0.43 g/L/h and biomass yield was 60 g CDW/L. The coefficient of lipid yield to glycerol consumption (Y L/gly) and the coefficient of lipid yield to biomass yield (Y L/X ) were equal to 0.1 and 0.4, respectively. These results indicate that lipids may be produced using renewable feedstock, thus providing a means of decreasing the cost of biodiesel production. Furthermore, using molasses for biomass production and recycling glycerol from the biodiesel industry should allow biolipids to be sustainably produced. |
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Mendeley readers
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Sweden | 2 | <1% |
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United States | 2 | <1% |
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Researcher | 30 | 13% |
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Student > Doctoral Student | 20 | 8% |
Other | 33 | 14% |
Unknown | 54 | 23% |
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Environmental Science | 7 | 3% |
Other | 16 | 7% |
Unknown | 68 | 29% |