Title |
Genome-wide mapping of promoter-anchored interactions with close to single-enhancer resolution
|
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Published in |
Genome Biology, August 2015
|
DOI | 10.1186/s13059-015-0727-9 |
Pubmed ID | |
Authors |
Pelin Sahlén, Ilgar Abdullayev, Daniel Ramsköld, Liudmila Matskova, Nemanja Rilakovic, Britta Lötstedt, Thomas J. Albert, Joakim Lundeberg, Rickard Sandberg |
Abstract |
Although the locations of promoters and enhancers have been identified in several cell types, we still have limited information on their connectivity. We developed HiCap, which combines a 4-cutter restriction enzyme Hi-C with sequence capture of promoter regions. Applying the method to mouse embryonic stem cells (mESCs), we identified promoter-anchored interactions involving 15,905 promoters and 71,984 distal regions. The distal regions were enriched for enhancer marks and transcription, and had a mean fragment size of only 699 bp - close to single-enhancer resolution. High-resolution maps of promoter-anchored interactions with HiCap will be important for detailed characterizations of chromatin interaction landscapes. |
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Denmark | 1 | 3% |
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Other | 0 | 0% |
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Demographic breakdown
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Members of the public | 11 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 3% |
Mendeley readers
Geographical breakdown
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United Kingdom | 2 | <1% |
Hungary | 1 | <1% |
Portugal | 1 | <1% |
Sweden | 1 | <1% |
France | 1 | <1% |
Switzerland | 1 | <1% |
China | 1 | <1% |
Denmark | 1 | <1% |
Other | 2 | <1% |
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Researcher | 61 | 25% |
Student > Bachelor | 24 | 10% |
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Student > Doctoral Student | 13 | 5% |
Other | 29 | 12% |
Unknown | 32 | 13% |
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Immunology and Microbiology | 4 | 2% |
Other | 13 | 5% |
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