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Exploration of serum- and cell culture-derived exosomes from dogs

Overview of attention for article published in BMC Veterinary Research, June 2018
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4 tweeters

Citations

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20 Dimensions

Readers on

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44 Mendeley
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Title
Exploration of serum- and cell culture-derived exosomes from dogs
Published in
BMC Veterinary Research, June 2018
DOI 10.1186/s12917-018-1509-x
Pubmed ID
Authors

Matias Aguilera-Rojas, Brit Badewien-Rentzsch, Johanna Plendl, Barbara Kohn, Ralf Einspanier

Abstract

Exosomes are defined as extracellular membrane vesicles, 30-150 nm in diameter, derived from all types of cells. They originate via endocytosis and then they are released through exocytosis to the extracellular space, being found in various biological fluids as well as in cell culture medium. In the last few years, exosomes have gained considerable scientific interest due to their potential use as biomarkers, especially in the field of cancer research. This report describes a method to isolate, quantify and identify serum- and cell culture-derived exosomes from dog samples, using small volumes (100 μL and 1 mL, respectively). Quantification and sizing of exosomes contained in serum and cell culture samples were assessed by utilizing nanoparticle tracking analysis, transmission electron microscopy and immunoelectron microscopy. Detected particles showed the normal size (30-150 nm) and morphology described for exosomes, as well as presence of the transmembrane protein CD63 known as exosomal marker. Based on a validated rapid isolation procedure of nanoparticles from small volumes of different types of dog samples, a characterization and exploration of intact exosomes, as well as facilitation for their analysis in downstream applications was introduced.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 20%
Student > Master 7 16%
Student > Bachelor 6 14%
Researcher 5 11%
Student > Doctoral Student 3 7%
Other 4 9%
Unknown 10 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 25%
Veterinary Science and Veterinary Medicine 10 23%
Agricultural and Biological Sciences 4 9%
Medicine and Dentistry 3 7%
Engineering 2 5%
Other 3 7%
Unknown 11 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 August 2018.
All research outputs
#8,458,564
of 13,488,230 outputs
Outputs from BMC Veterinary Research
#909
of 1,985 outputs
Outputs of similar age
#161,388
of 269,904 outputs
Outputs of similar age from BMC Veterinary Research
#1
of 2 outputs
Altmetric has tracked 13,488,230 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,985 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,904 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them