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X Demographics
Mendeley readers
Attention Score in Context
| Title |
High-throughput and quantitative genome-wide messenger RNA sequencing for molecular phenotyping
|
|---|---|
| Published in |
BMC Genomics, August 2015
|
| DOI | 10.1186/s12864-015-1788-6 |
| Pubmed ID | |
| Authors |
John E. Collins, Neha Wali, Ian M. Sealy, James A. Morris, Richard J. White, Steven R. Leonard, David K. Jackson, Matthew C. Jones, Nathalie C. Smerdon, Jorge Zamora, Christopher M. Dooley, Samantha N. Carruthers, Jeffrey C. Barrett, Derek L. Stemple, Elisabeth M. Busch-Nentwich |
| Abstract |
We present a genome-wide messenger RNA (mRNA) sequencing technique that converts small amounts of RNA from many samples into molecular phenotypes. It encompasses all steps from sample preparation to sequence analysis and is applicable to baseline profiling or perturbation measurements. Multiplex sequencing of transcript 3' ends identifies differential transcript abundance independent of gene annotation. We show that increasing biological replicate number while maintaining the total amount of sequencing identifies more differentially abundant transcripts. This method can be implemented on polyadenylated RNA from any organism with an annotated reference genome and in any laboratory with access to Illumina sequencing. |
X Demographics
The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 11% |
| Brazil | 1 | 11% |
| Germany | 1 | 11% |
| France | 1 | 11% |
| Unknown | 5 | 56% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 56% |
| Scientists | 4 | 44% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 2% |
| Switzerland | 1 | 2% |
| Netherlands | 1 | 2% |
| Chile | 1 | 2% |
| Sweden | 1 | 2% |
| Benin | 1 | 2% |
| Denmark | 1 | 2% |
| Unknown | 47 | 87% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 31% |
| Student > Ph. D. Student | 13 | 24% |
| Student > Master | 6 | 11% |
| Student > Bachelor | 5 | 9% |
| Other | 4 | 7% |
| Other | 5 | 9% |
| Unknown | 4 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 54% |
| Biochemistry, Genetics and Molecular Biology | 10 | 19% |
| Engineering | 3 | 6% |
| Computer Science | 2 | 4% |
| Environmental Science | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 7 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2021.
All research outputs
#2,098,541
of 22,818,766 outputs
Outputs from BMC Genomics
#602
of 10,654 outputs
Outputs of similar age
#28,764
of 264,147 outputs
Outputs of similar age from BMC Genomics
#13
of 245 outputs
Altmetric has tracked 22,818,766 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,654 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,147 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 245 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.