Title |
Activin A increases phagocytosis of Escherichia coli K1 by primary murine microglial cells activated by toll-like receptor agonists
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Published in |
Journal of Neuroinflammation, June 2018
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DOI | 10.1186/s12974-018-1209-2 |
Pubmed ID | |
Authors |
Catharina Diesselberg, Sandra Ribes, Jana Seele, Annika Kaufmann, Sandra Redlich, Stephanie Bunkowski, Uwe-Karsten Hanisch, Uwe Michel, Roland Nau, Sandra Schütze |
Abstract |
Bacterial meningitis is associated with high mortality and long-term neurological sequelae. Increasing the phagocytic activity of microglia could improve the resistance of the CNS against infections. We studied the influence of activin A, a member of the TGF-β family with known immunoregulatory and neuroprotective effects, on the functions of microglial cells in vitro. Primary murine microglial cells were treated with activin A (0.13 ng/ml-13 μg/ml) alone or in combination with agonists of TLR2, 4, and 9. Phagocytosis of Escherichia coli K1 as well as release of TNF-α, IL-6, CXCL1, and NO was assessed. Activin A dose-dependently enhanced the phagocytosis of Escherichia coli K1 by microglial cells activated by agonists of TLR2, 4, and 9 without further increasing NO and proinflammatory cytokine release. Cell viability of microglial cells was not affected by activin A. Priming of microglial cells with activin A could increase the elimination of bacteria in bacterial CNS infections. This preventive strategy could improve the resistance of the brain to infections, particularly in elderly and immunocompromised patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 29% |
Student > Doctoral Student | 3 | 14% |
Student > Bachelor | 2 | 10% |
Student > Master | 2 | 10% |
Unspecified | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 19% |
Immunology and Microbiology | 3 | 14% |
Medicine and Dentistry | 2 | 10% |
Neuroscience | 2 | 10% |
Agricultural and Biological Sciences | 1 | 5% |
Other | 3 | 14% |
Unknown | 6 | 29% |