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Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Overview of attention for article published in Plant Methods, June 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (64th percentile)

Mentioned by

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7 tweeters

Citations

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14 Dimensions

Readers on

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21 Mendeley
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Title
Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips
Published in
Plant Methods, June 2018
DOI 10.1186/s13007-018-0312-9
Pubmed ID
Authors

Min-Rui Wang, Wen Yang, Lei Zhao, Jing-Wei Li, Ke Liu, Jing-Wei Yu, Yun-Feng Wu, Qiao-Chun Wang

Abstract

Preservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus would largely assist these studies. The present study reported a novel biotechnology allowing cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips. Following cryopreservation by droplet-vitrification or encapsulation-dehydration, about 62-67% of shoot regrowth and 100% of ASGV cryopreservation were obtained. Although shoot proliferation and virus concentration were reduced in cryopreserved diseased shoots after 8 weeks of shoot regeneration, continuous subculture for 4 times (16 weeks) increased shoot proliferation and virus concentration to comparative levels as those produced by shoot tip culture (as a control to shoot tip cryopreservation). Cryopreserved ASGV was efficiently transmitted to a woody plant by micrografting and to a herbaceous indicator by mechanical inoculation. Gene sequencing in three fragments of ASGV genome including coat protein and movement protein showed that cryopreserved ASGV shared 99.87% nucleotide identities with shoot tip culture-preserved virus, indicating cryopreserved virus is genetically stable. The present study demonstrates ASGV, a representative virus that can infect meristematic cells of shoot tips, can be efficiently cryopreserved in shoot tips. To the best of our knowledge, this is the first report on plant virus cryopreservation in living tissues, and has great potential applications to long-term preservation of plant viruses.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 29%
Student > Ph. D. Student 6 29%
Student > Bachelor 2 10%
Researcher 2 10%
Other 1 5%
Other 1 5%
Unknown 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 38%
Biochemistry, Genetics and Molecular Biology 2 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Arts and Humanities 1 5%
Nursing and Health Professions 1 5%
Other 3 14%
Unknown 5 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2020.
All research outputs
#5,460,282
of 18,663,462 outputs
Outputs from Plant Methods
#317
of 891 outputs
Outputs of similar age
#101,759
of 290,572 outputs
Outputs of similar age from Plant Methods
#1
of 1 outputs
Altmetric has tracked 18,663,462 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 891 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 290,572 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them