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Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling

Overview of attention for article published in Lipids in Health and Disease, August 2015
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Title
Lipid changes within the epidermis of living skin equivalents observed across a time-course by MALDI-MS imaging and profiling
Published in
Lipids in Health and Disease, August 2015
DOI 10.1186/s12944-015-0089-z
Pubmed ID
Authors

Christopher A. Mitchell, Heather Long, Michael Donaldson, Simona Francese, Malcolm R Clench

Abstract

Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. Here, its application to the study of the distribution of lipids in sections of reconstructed living skin equivalents during their development and maturation is described. Living skin equivalent (LSE) samples were obtained at 14 days development, re-suspended in maintenance medium and incubated for 24 h after delivery. The medium was then changed, the LSE re-incubated and samples taken at 4, 6 and 24 h time points. Mass spectra and mass spectral images were recorded from 12 μm sections of the LSE taken at each time point for comparison using matrix assisted laser desorption ionisation mass spectrometry. A large number of lipid species were identified in the LSE via accurate mass-measurement MS and MSMS experiments carried out directly on the tissue sections. MS images acquired at a spatial resolution of 50 μm × 50 μm showed the distribution of identified lipids within the developing LSE and changes in their distribution with time. In particular development of an epidermal layer was observable as a compaction of the distribution of phosphatidylcholine species. MSI can be used to study changes in lipid composition in LSE. Determination of the changes in lipid distribution during the maturation of the LSE will assist in the identification of treatment responses in future investigations.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Austria 1 1%
Unknown 65 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 30%
Researcher 13 19%
Student > Master 9 13%
Student > Doctoral Student 5 7%
Other 4 6%
Other 5 7%
Unknown 11 16%
Readers by discipline Count As %
Chemistry 13 19%
Biochemistry, Genetics and Molecular Biology 11 16%
Agricultural and Biological Sciences 9 13%
Medicine and Dentistry 6 9%
Pharmacology, Toxicology and Pharmaceutical Science 5 7%
Other 8 12%
Unknown 15 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2015.
All research outputs
#20,286,650
of 22,821,814 outputs
Outputs from Lipids in Health and Disease
#1,204
of 1,450 outputs
Outputs of similar age
#220,914
of 264,159 outputs
Outputs of similar age from Lipids in Health and Disease
#27
of 35 outputs
Altmetric has tracked 22,821,814 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,450 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.