Title |
RNA-Seq improves annotation of protein-coding genes in the cucumber genome
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Published in |
BMC Genomics, November 2011
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DOI | 10.1186/1471-2164-12-540 |
Pubmed ID | |
Authors |
Zhen Li, Zhonghua Zhang, Pengcheng Yan, Sanwen Huang, Zhangjun Fei, Kui Lin |
Abstract |
As more and more genomes are sequenced, genome annotation becomes increasingly important in bridging the gap between sequence and biology. Gene prediction, which is at the center of genome annotation, usually integrates various resources to compute consensus gene structures. However, many newly sequenced genomes have limited resources for gene predictions. In an effort to create high-quality gene models of the cucumber genome (Cucumis sativus var. sativus), based on the EVidenceModeler gene prediction pipeline, we incorporated the massively parallel complementary DNA sequencing (RNA-Seq) reads of 10 cucumber tissues into EVidenceModeler. We applied the new pipeline to the reassembled cucumber genome and included a comparison between our predicted protein-coding gene sets and a published set. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 9 | 5% |
Sweden | 3 | 2% |
Italy | 2 | 1% |
United Kingdom | 2 | 1% |
Canada | 2 | 1% |
India | 1 | <1% |
Czechia | 1 | <1% |
Brazil | 1 | <1% |
Mexico | 1 | <1% |
Other | 6 | 3% |
Unknown | 163 | 85% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 57 | 30% |
Student > Ph. D. Student | 48 | 25% |
Student > Master | 22 | 12% |
Student > Doctoral Student | 13 | 7% |
Student > Bachelor | 11 | 6% |
Other | 23 | 12% |
Unknown | 17 | 9% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 32 | 17% |
Computer Science | 4 | 2% |
Social Sciences | 2 | 1% |
Earth and Planetary Sciences | 2 | 1% |
Other | 2 | 1% |
Unknown | 20 | 10% |